Advances in Aptamer-Based Conjugate Recognition Techniques for the Detection of Small Molecules in Food

Overview

Journal Foods

Specialty Biotechnology

Date 2024 Jun 19

PMID 38890976

Authors

Xin Deng

Biao Ma

Yunfei Gong

Jiali Li

Yuxin Zhou

Tianran Xu

Peiying Hao

Kai Sun

Zhiyong Lv

Xiaoping Yu

Mingzhou Zhang

Affiliations

Soon will be listed here.

Abstract

Small molecules are significant risk factors for causing food safety issues, posing serious threats to human health. Sensitive screening for hazards is beneficial for enhancing public security. However, traditional detection methods are unable to meet the requirements for the field screening of small molecules. Therefore, it is necessary to develop applicable methods with high levels of sensitivity and specificity to identify the small molecules. Aptamers are short-chain nucleic acids that can specifically bind to small molecules. By utilizing aptamers to enhance the performance of recognition technology, it is possible to achieve high selectivity and sensitivity levels when detecting small molecules. There have been several varieties of aptamer target recognition techniques developed to improve the ability to detect small molecules in recent years. This review focuses on the principles of detection platforms, classifies the conjugating methods between small molecules and aptamers, summarizes advancements in aptamer-based conjugate recognition techniques for the detection of small molecules in food, and seeks to provide emerging powerful tools in the field of point-of-care diagnostics.

References

Li R, Liu L, Zhu H, Li Z . Synthesis of gold-palladium nanowaxberries/dodecylamine-functionalized graphene quantum dots-graphene micro-aerogel for voltammetric determination of peanut allergen Ara h 1. Anal Chim Acta. 2018; 1008:38-47. DOI: 10.1016/j.aca.2018.01.031. View

Sheng Y, Liang J, Xie J . Indirect Competitive Determination of Tetracycline Residue in Honey Using an Ultrasensitive Gold-Nanoparticle-Linked Aptamer Assay. Molecules. 2020; 25(9). PMC: 7249119. DOI: 10.3390/molecules25092144. View

Huang R, Xiong L, Chai H, Fu J, Lu Z, Yu L . Sensitive colorimetric detection of ochratoxin A by a dual-functional Au/FeO nanohybrid-based aptasensor. RSC Adv. 2022; 9(66):38590-38596. PMC: 9075840. DOI: 10.1039/c9ra07899a. View

Lerga T, Jauset-Rubio M, Skouridou V, Bashammakh A, El-Shahawi M, Alyoubi A . High Affinity Aptamer for the Detection of the Biogenic Amine Histamine. Anal Chem. 2019; 91(11):7104-7111. DOI: 10.1021/acs.analchem.9b00075. View

Tang T, Deng J, Zhang M, Shi G, Zhou T . Quantum dot-DNA aptamer conjugates coupled with capillary electrophoresis: A universal strategy for ratiometric detection of organophosphorus pesticides. Talanta. 2015; 146:55-61. DOI: 10.1016/j.talanta.2015.08.023. View

Bagheri A, Ghaedi M . Magnetic metal organic framework for pre-concentration of ampicillin from cow milk samples. J Pharm Anal. 2020; 10(4):365-375. PMC: 7474158. DOI: 10.1016/j.jpha.2020.02.006. View

Liu X, Lu Q, Chen S, Wang F, Hou J, Xu Z . Selection and Identification of Novel Aptamers Specific for Clenbuterol Based on ssDNA Library Immobilized SELEX and Gold Nanoparticles Biosensor. Molecules. 2018; 23(9). PMC: 6225122. DOI: 10.3390/molecules23092337. View

Chen Q, Zuo J, He X, Mo X, Tong P, Zhang L . Enhanced fluorescence of terbium with thiabendazole and application in determining trace amounts of terbium and thiabendazole. Talanta. 2016; 162:540-546. DOI: 10.1016/j.talanta.2016.10.036. View

Schreiner S, Shudy D, Hatch A, Opdahl A, Whitman L, Petrovykh D . Controlled and efficient hybridization achieved with DNA probes immobilized solely through preferential DNA-substrate interactions. Anal Chem. 2010; 82(7):2803-10. DOI: 10.1021/ac902765g. View

10.

Zhou W, Kong W, Dou X, Zhao M, Ouyang Z, Yang M . An aptamer based lateral flow strip for on-site rapid detection of ochratoxin A in Astragalus membranaceus. J Chromatogr B Analyt Technol Biomed Life Sci. 2016; 1022:102-108. DOI: 10.1016/j.jchromb.2016.04.016. View

11.

Huang Q, Zhao Z, Nie D, Jiang K, Guo W, Fan K . Molecularly Imprinted Poly(thionine)-Based Electrochemical Sensing Platform for Fast and Selective Ultratrace Determination of Patulin. Anal Chem. 2019; 91(6):4116-4123. DOI: 10.1021/acs.analchem.8b05791. View

12.

Yang C, Lates V, Prieto-Simon B, Marty J, Yang X . Aptamer-DNAzyme hairpins for biosensing of Ochratoxin A. Biosens Bioelectron. 2012; 32(1):208-12. DOI: 10.1016/j.bios.2011.12.011. View

13.

Qiang L, Zhang Y, Guo X, Gao Y, Han Y, Sun J . A rapid and ultrasensitive colorimetric biosensor based on aptamer functionalized Au nanoparticles for detection of saxitoxin. RSC Adv. 2022; 10(26):15293-15298. PMC: 9052278. DOI: 10.1039/d0ra01231a. View

14.

Zou Y, Griveau S, Ringuede A, Bedioui F, Richard C, Slim C . Functionalized Multi-Walled Carbon Nanotube-Based Aptasensors for Diclofenac Detection. Front Chem. 2022; 9:812909. PMC: 8818859. DOI: 10.3389/fchem.2021.812909. View

15.

Mermiga E, Pagkali V, Kokkinos C, Economou A . An Aptamer-Based Lateral Flow Biosensor for Low-Cost, Rapid and Instrument-Free Detection of Ochratoxin A in Food Samples. Molecules. 2023; 28(24). PMC: 10746035. DOI: 10.3390/molecules28248135. View

16.

Pei H, Li F, Wan Y, Wei M, Liu H, Su Y . Designed diblock oligonucleotide for the synthesis of spatially isolated and highly hybridizable functionalization of DNA-gold nanoparticle nanoconjugates. J Am Chem Soc. 2012; 134(29):11876-9. DOI: 10.1021/ja304118z. View

17.

Zhu C, Zhang G, Huang Y, Yang S, Ren S, Gao Z . Dual-competitive lateral flow aptasensor for detection of aflatoxin B in food and feedstuffs. J Hazard Mater. 2017; 344:249-257. DOI: 10.1016/j.jhazmat.2017.10.026. View

18.

Borghei Y, Hosseinkhani S . Aptamer-based colorimetric determination of early-stage apoptotic cells via the release of cytochrome c from mitochondria and by exploiting silver/platinum alloy nanoclusters as a peroxidase mimic. Mikrochim Acta. 2019; 186(12):845. DOI: 10.1007/s00604-019-3977-5. View

19.

Liu Y, OuYang Q, Li H, Chen M, Zhang Z, Chen Q . Turn-On Fluoresence Sensor for Hg in Food Based on FRET between Aptamers-Functionalized Upconversion Nanoparticles and Gold Nanoparticles. J Agric Food Chem. 2018; 66(24):6188-6195. DOI: 10.1021/acs.jafc.8b00546. View

20.

Zeng Y, Liang D, Zheng P, Peng T, Sun S, Mari G . Immunochromatographic fluorometric determination of clenbuterol with enhanced sensitivity. Mikrochim Acta. 2019; 186(4):225. DOI: 10.1007/s00604-019-3326-8. View