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Assignment of the 13C Nuclear Magnetic Resonance Spectrum of a Short DNA-duplex with 1H-detected Two-dimensional Heteronuclear Correlation Spectroscopy

Overview
Journal Nucleic Acids Res
Publisher Oxford University Press
Specialty Biochemistry
Date 1987 Jan 12
PMID 3822804
Citations 4
Authors
W Leupin
G Wagner
W A Denny
K Wuthrich
Affiliations
Soon will be listed here.
Abstract

Proton-detected 1H-13C heteronuclear correlated spectroscopy [( 1H,13C]-COSY) was used to establish relations between the carbon-13 and proton nuclear magnetic resonance chemical shifts in the hexadeoxynucleoside pentaphosphate d-(GCATGC)2. Using the previously established sequence-specific proton NMR assignments, sequence-specific assignments were thus obtained for nearly all proton-bearing carbons. This approach offers a new criterion for distinguishing between the proton NMR lines of purines and pyrimidines, based on the different proton-carbon-13 coupling constants. Furthermore, the adenine ring carbon 2 has a unique carbon-13 chemical shift, which enables a straightforward identification of the adenine C2H resonances by [1H,13C]-COSY.

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