Comparative In Vitro Dissolution Assessment of Calcined and Uncalcined Hydroxyapatite Using Differences in Bioresorbability and Biomineralization

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2024 Jan 11

PMID 38203791

Authors

Woo Young Jang

Jae Chul Pyun

Jeong Ho Chang

Affiliations

Soon will be listed here.

Abstract

This study reports the effect of the not-calcining process on the bioresorption and biomineralization of hydroxyapatite through in vitro dissolution assessment. The prepared calcined hydroxyapatite (c-HAp) and uncalcined hydroxyapatite (unc-HAp) have a particle size of 2 μm and 13 μm, surface areas of 4.47 m/g and 108.08 m/g, and a Ca/P ratio of 1.66 and 1.52, respectively. In vitro dissolution assessments of c-HAp and unc-HAp were performed for 20 days at 37 °C in a citric acid buffer according to ISO 10993-14. During the dissolution, the c-HAp and unc-HAp confirmed an increase in weight, and the calcium and phosphorous ions were rapidly released. The calcium ions released from c-HAp formed rod-shaped particles with a longer and thinner morphology, while in unc-HAp, they appeared thicker and shorter. In the ICP-OES results, the concentrations of calcium elements were initially increased and then decreased by this formation. The rod-shaped particles identified as calcium citrate (Ca-citrate) through the XRD pattern. The calcium content of Ca-citrate particles from unc-HAp was higher than that from c-HAp. The unc-HAp demonstrated non-toxic properties in a cytotoxicity evaluation. Therefore, due to its higher bioresorption and biomineralization, unc-HAp exhibits enhanced biocompatibility compared to c-HAp.

Citing Articles

Bioresorption and Biomineralization of S53P4 Bioactive Glass in Neutral Tris Buffer and Citric Acid Solution.

Yoo K, Jang W, Chang J ACS Omega. 2024; 9(43):43678-43688.

PMID: 39493975 PMC: 11525500. DOI: 10.1021/acsomega.4c06020.

References

SHIKINAMI Y, Okuno M . Bioresorbable devices made of forged composites of hydroxyapatite (HA) particles and poly-L-lactide (PLLA): Part I. Basic characteristics. Biomaterials. 1999; 20(9):859-77. DOI: 10.1016/s0142-9612(98)00241-5. View

Ishizuka S, Dong Q, Ngo H, Bai Y, Sha J, Toda E . Bioactive Regeneration Potential of the Newly Developed Uncalcined/Unsintered Hydroxyapatite and Poly-l-Lactide-Co-Glycolide Biomaterial in Maxillofacial Reconstructive Surgery: An In Vivo Preliminary Study. Materials (Basel). 2021; 14(9). PMC: 8126161. DOI: 10.3390/ma14092461. View

Noorani T, Luddin N, Ab Rahman I, Masudi S . In Vitro Cytotoxicity Evaluation of Novel Nano-Hydroxyapatite-Silica Incorporated Glass Ionomer Cement. J Clin Diagn Res. 2017; 11(4):ZC105-ZC109. PMC: 5449899. DOI: 10.7860/JCDR/2017/24753.9739. View

Li J, Deng C, Liang W, Kang F, Bai Y, Ma B . Mn-containing bioceramics inhibit osteoclastogenesis and promote osteoporotic bone regeneration via scavenging ROS. Bioact Mater. 2021; 6(11):3839-3850. PMC: 8050801. DOI: 10.1016/j.bioactmat.2021.03.039. View

Zhang Y, Shu T, Wang S, Liu Z, Cheng Y, Li A . The Osteoinductivity of Calcium Phosphate-Based Biomaterials: A Tight Interaction With Bone Healing. Front Bioeng Biotechnol. 2022; 10:911180. PMC: 9149242. DOI: 10.3389/fbioe.2022.911180. View

Lu X, Kolzow J, Chen R, Du J . Effect of solution condition on hydroxyapatite formation in evaluating bioactivity of BO containing 45S5 bioactive glasses. Bioact Mater. 2019; 4:207-214. PMC: 6555879. DOI: 10.1016/j.bioactmat.2019.05.002. View

Kottegoda N, Sandaruwan C, Priyadarshana G, Siriwardhana A, Rathnayake U, Berugoda Arachchige D . Urea-Hydroxyapatite Nanohybrids for Slow Release of Nitrogen. ACS Nano. 2017; 11(2):1214-1221. DOI: 10.1021/acsnano.6b07781. View

SHIKINAMI Y, Okuno M . Bioresorbable devices made of forged composites of hydroxyapatite (HA) particles and poly L-lactide (PLLA). Part II: practical properties of miniscrews and miniplates. Biomaterials. 2001; 22(23):3197-211. DOI: 10.1016/s0142-9612(01)00072-2. View

Hong M, Son J, Kim K, Han M, Oh D, Lee Y . Drug-loaded porous spherical hydroxyapatite granules for bone regeneration. J Mater Sci Mater Med. 2011; 22(2):349-55. DOI: 10.1007/s10856-010-4197-z. View

10.

Liu H, Yazici H, Ergun C, Webster T, Bermek H . An in vitro evaluation of the Ca/P ratio for the cytocompatibility of nano-to-micron particulate calcium phosphates for bone regeneration. Acta Biomater. 2008; 4(5):1472-9. DOI: 10.1016/j.actbio.2008.02.025. View

11.

Mohd Puad N, Koshy P, Abdullah H, Idris M, Lee T . Syntheses of hydroxyapatite from natural sources. Heliyon. 2019; 5(5):e01588. PMC: 6507053. DOI: 10.1016/j.heliyon.2019.e01588. View

12.

Lee M, Hwang H, Oh S, Roshanzadeh A, Kim J, Song J . Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression. Exp Mol Med. 2018; 50(11):1-16. PMC: 6215840. DOI: 10.1038/s12276-018-0170-6. View

13.

Pellegrino E, BILTZ R . Bone carbonate and the Ca to P molar ratio. Nature. 1968; 219(5160):1261-2. DOI: 10.1038/2191261a0. View

14.

Jiang Q, Wang L, Liu Z, Su J, Tang Y, Tan P . Canine ACL reconstruction with an injectable hydroxyapatite/collagen paste for accelerated healing of tendon-bone interface. Bioact Mater. 2022; 20:1-15. PMC: 9123091. DOI: 10.1016/j.bioactmat.2022.05.003. View

15.

Wang H, Lee J, Moursi A, Lannutti J . Ca/P ratio effects on the degradation of hydroxyapatite in vitro. J Biomed Mater Res A. 2003; 67(2):599-608. DOI: 10.1002/jbm.a.10538. View

16.

Yang H, Zeng H, Hao L, Zhao N, Du C, Liao H . Effects of hydroxyapatite microparticle morphology on bone mesenchymal stem cell behavior. J Mater Chem B. 2020; 2(29):4703-4710. DOI: 10.1039/c4tb00424h. View

17.

Kaygili O, Keser S, Kom M, Bulut N, Dorozhkin S . The effect of simulating body fluid on the structural properties of hydroxyapatite synthesized in the presence of citric acid. Prog Biomater. 2016; 5(3-4):173-182. PMC: 5301803. DOI: 10.1007/s40204-016-0055-5. View

18.

Sukegawa S, Kanno T, Katase N, Shibata A, Takahashi Y, Furuki Y . Clinical Evaluation of an Unsintered Hydroxyapatite/Poly-L-Lactide Osteoconductive Composite Device for the Internal Fixation of Maxillofacial Fractures. J Craniofac Surg. 2016; 27(6):1391-7. PMC: 5023762. DOI: 10.1097/SCS.0000000000002828. View

19.

Le Gars Santoni B, Niggli L, Dolder S, Loeffel O, Sblendorio G, Heuberger R . Effect of minor amounts of β-calcium pyrophosphate and hydroxyapatite on the physico-chemical properties and osteoclastic resorption of β-tricalcium phosphate cylinders. Bioact Mater. 2021; 10:222-235. PMC: 8636826. DOI: 10.1016/j.bioactmat.2021.09.003. View

20.

Alorku K, Manoj M, Yuan A . A plant-mediated synthesis of nanostructured hydroxyapatite for biomedical applications: a review. RSC Adv. 2022; 10(67):40923-40939. PMC: 9057773. DOI: 10.1039/d0ra08529d. View