A Combined Gas and Liquid Chromatographic Approach for Quality Evaluation of Saffron-Based Food Supplements

Overview

Journal Foods

Specialty Biotechnology

Date 2023 Nov 25

PMID 38002129

Authors

Adal Mena-Garcia

Maria L Sanz

Marina Diez-Municio

Ana I Ruiz-Matute

Affiliations

Soon will be listed here.

Abstract

Considering the interest in the bioactive properties of saffron ( L.), as well as its limited production and high price, saffron-based food supplements (SFS) are highly susceptible to adulteration. However, their complex composition and the wide variety of potential fraudulent practices make the comprehensive assessment of SFS quality a challenging task that has been scarcely addressed. To that aim, a new multianalytical strategy based on gas chromatography coupled to mass spectrometry (GC-MS) and liquid chromatography with diode array detection coupled to mass spectrometry (HPLC-DAD-MS) was developed and validated in order to detect different frauds affecting SFS. Dried saffron stigmas and a commercial standardized saffron extract (affron) were selected as reference samples (RS) to obtain an authenticity profile, which was further used to evaluate the quality of 17 SFS. Up to 17 crocins and crocetins, 5 kaempferol glycosides, picrocrocin (determined for the first time by GC-MS), safranal, furanone and isophorone-related compounds were determined in RS. Safranal and crocins were identified in all SFS except for one sample. However, discrepancies with the content declared were detected in 65% of the cases. Moreover, this multianalytical methodology also allowed identifying undeclared additives and the non-declared addition of vegetable sources other than saffron.

References

Husaini A, Jan K, Wani G . Saffron: A potential drug-supplement for severe acute respiratory syndrome coronavirus (COVID) management. Heliyon. 2021; 7(5):e07068. PMC: 8118646. DOI: 10.1016/j.heliyon.2021.e07068. View

Guijarro-Diez M, Nozal L, Marina M, Crego A . Metabolomic fingerprinting of saffron by LC/MS: novel authenticity markers. Anal Bioanal Chem. 2015; 407(23):7197-213. DOI: 10.1007/s00216-015-8882-0. View

Dwyer J, Coates P, Smith M . Dietary Supplements: Regulatory Challenges and Research Resources. Nutrients. 2018; 10(1). PMC: 5793269. DOI: 10.3390/nu10010041. View

Moshiri M, Vahabzadeh M, Hosseinzadeh H . Clinical Applications of Saffron (Crocus sativus) and its Constituents: A Review. Drug Res (Stuttg). 2014; 65(6):287-95. DOI: 10.1055/s-0034-1375681. View

Aiello D, Siciliano C, Mazzotti F, Di Donna L, Athanassopoulos C, Napoli A . A rapid MALDI MS/MS based method for assessing saffron (Crocus sativus L.) adulteration. Food Chem. 2019; 307:125527. DOI: 10.1016/j.foodchem.2019.125527. View

Bilia A, Bergonzi M . The G115 standardized ginseng extract: an example for safety, efficacy, and quality of an herbal medicine. J Ginseng Res. 2020; 44(2):179-193. PMC: 7031746. DOI: 10.1016/j.jgr.2019.06.003. View

Neves D, Caldas E . Determination of caffeine and identification of undeclared substances in dietary supplements and caffeine dietary exposure assessment. Food Chem Toxicol. 2017; 105:194-202. DOI: 10.1016/j.fct.2017.03.063. View

Sabatino L, Scordino M, Gargano M, Belligno A, Traulo P, Gagliano G . HPLC/PDA/ESI-MS evaluation of saffron (Crocus sativus L.) adulteration. Nat Prod Commun. 2012; 6(12):1873-6. View

Mena-Garcia A, Bellaizac-Riascos A, Rada-Mendoza M, Chito-Trujillo D, Ruiz-Matute A, Sanz M . Quality Evaluation of Dietary Supplements for Weight Loss Based on . Nutrients. 2022; 14(15). PMC: 9332841. DOI: 10.3390/nu14153077. View

10.

Ruiz-Aceituno L, Carrero-Carralero C, Ruiz-Matute A, Ramos L, Sanz M, Martinez-Castro I . Characterization of cyclitol glycosides by gas chromatography coupled to mass spectrometry. J Chromatogr A. 2017; 1484:58-64. DOI: 10.1016/j.chroma.2017.01.001. View

11.

Semen S, Mercan S, Yayla M, Acikkol M . Elemental composition of green coffee and its contribution to dietary intake. Food Chem. 2016; 215:92-100. DOI: 10.1016/j.foodchem.2016.07.176. View

12.

Mena-Garcia A, Herrero-Gutierrez D, Sanz M, Diez-Municio M, Ruiz-Matute A . Fingerprint of Characteristic Saffron Compounds as Novel Standardization of Commercial Extracts. Foods. 2023; 12(8). PMC: 10137349. DOI: 10.3390/foods12081634. View

13.

Zuluaga A, Mena-Garcia A, Chito-Trujillo D, Rada-Mendoza M, Sanz M, Ruiz-Matute A . Development of a microwave-assisted extraction method for the recovery of bioactive inositols from lettuce (Lactuca sativa) byproducts. Electrophoresis. 2020; 41(20):1804-1811. DOI: 10.1002/elps.202000201. View

14.

Mena-Garcia A, Ruiz-Matute A, Soria A, Sanz M . A multi-analytical strategy for evaluation of quality and authenticity of artichoke food supplements for overweight control. J Chromatogr A. 2021; 1647:462102. DOI: 10.1016/j.chroma.2021.462102. View

15.

Liu J, Chen N, Yang J, Yang B, Ouyang Z, Wu C . An integrated approach combining HPLC, GC/MS, NIRS, and chemometrics for the geographical discrimination and commercial categorization of saffron. Food Chem. 2018; 253:284-292. DOI: 10.1016/j.foodchem.2018.01.140. View

16.

Amanpour A, Sonmezdag A, Kelebek H, Selli S . GC-MS-olfactometric characterization of the most aroma-active components in a representative aromatic extract from Iranian saffron (Crocus sativus L.). Food Chem. 2015; 182:251-6. DOI: 10.1016/j.foodchem.2015.03.005. View

17.

Alonso G, Salinas M, Garijo J . Method to determine the authenticity of aroma of saffron (Crocus sativus L.). J Food Prot. 1998; 61(11):1525-8. DOI: 10.4315/0362-028x-61.11.1525. View

18.

Cid-Perez T, Nevarez-Moorillon G, Ochoa-Velasco C, Navarro-Cruz A, Hernandez-Carranza P, Avila-Sosa R . The Relation between Drying Conditions and the Development of Volatile Compounds in Saffron (). Molecules. 2021; 26(22). PMC: 8621395. DOI: 10.3390/molecules26226954. View

19.

Ruiz-Aceituno L, Rodriguez-Sanchez S, Ruiz-Matute A, Ramos L, Soria A, Sanz M . Optimisation of a biotechnological procedure for selective fractionation of bioactive inositols in edible legume extracts. J Sci Food Agric. 2013; 93(11):2797-803. DOI: 10.1002/jsfa.6103. View

20.

Cossignani L, Urbani E, Simonetti M, Maurizi A, Chiesi C, Blasi F . Characterisation of secondary metabolites in saffron from central Italy (Cascia, Umbria). Food Chem. 2013; 143:446-51. DOI: 10.1016/j.foodchem.2013.08.020. View