SARS-CoV-2 NSP12 Associates with TRiC and the P323L Substitution Acts As a Host Adaption

Overview

Journal J Virol

Publisher American Society for Microbiology

Date 2023 Nov 6

PMID 37929963

Authors

Muhannad Alruwaili

Stuart Armstrong

Tessa Prince

Maximillian Erdmann

David A Matthews

Lisa Luu

Andrew Davidson

Waleed Aljabr

Julian A Hiscox

Affiliations

Soon will be listed here.

Abstract

SARS-CoV-2 has caused a worldwide health and economic crisis. During the course of the pandemic, genetic changes occurred in the virus, which have resulted in new properties of the virus-particularly around gains in transmission and the ability to partially evade either natural or vaccine-acquired immunity. Some of these viruses have been labeled Variants of Concern (VoCs). At the root of all VoCs are two mutations, one in the viral spike protein that has been very well characterized and the other in the virus polymerase (NSP12). This is the viral protein responsible for replicating the genome. We show that NSP12 associates with host cell proteins that act as a scaffold to facilitate the function of this protein. Furthermore, we found that different variants of NSP12 interact with host cell proteins in subtle and different ways, which affect function.

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