The Impact of Leucine Supplementation on Body Composition and Glucose Tolerance Following Energy Restriction: an 8-week RCT in Adults at Risk of the Metabolic Syndrome

Overview

Journal Eur J Clin Nutr

Specialty Nutritional Sciences

Date 2023 Nov 4

PMID 37923932

Authors

Kaveri Pathak

Yun Zhao

Emily K Calton

Anthony P James

Philip Newsholme

Jill Sherriff

Mario J Soares

Affiliations

Soon will be listed here.

Abstract

Background: L-Leucine (Leu) supplementation may benefit fat-free mass (FFM) per se and glucose metabolism.

Objectives: To determine whether Leu supplementation during energy restriction blunted the loss of FFM, enhanced the loss of fat mass (FM) and improved glucose tolerance.

Design: Thirty-seven adults, aged 20-65 years, with increased waist circumference and at least one other metabolic syndrome (MetS) component, were selected. We employed a two-arm parallel, double blind, randomized control trial (RCT) design. Participants were randomly assigned to an intervention group (leucine - 3 g/d) or placebo (lactose - 2.67 g/d), while following an individualised energy restricted diet for an 8-week period. Detailed body composition (DEXA), oral glucose tolerance test (OGTT), insulin and components of MetS were measured before and after the trial. Analysis of covariance (ANCOVA) assessed the effect of Leu on an intention-to-treat (ITT) principle. Bootstrapping method with 1000 bootstrap samples was used to derive parameter estimates, standard errors, p-values, and 95% confidence intervals for all outcomes.

Results: Adjusted for baseline values and other covariates, FFM (p = 0.045) and lean tissue mass (LTM) (p = 0.050) were significantly higher following Leu. These outcomes were modified by a significant treatment x sex interaction that indicated Leu had the greater effect in men. However, on adjustment for body composition changes, there was no difference in insulin sensitivity, oral glucose tolerance, or change in MetS components following Leu.

Conclusion: Short-term leucine supplementation during energy restriction resulted in a greater preservation of FFM and LTM particularly in men, but did not impact glucose metabolism.

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References

Park Y, Zhu S, Palaniappan L, Heshka S, Carnethon M, Heymsfield S . The metabolic syndrome: prevalence and associated risk factor findings in the US population from the Third National Health and Nutrition Examination Survey, 1988-1994. Arch Intern Med. 2003; 163(4):427-36. PMC: 3146257. DOI: 10.1001/archinte.163.4.427. View

Andreadis E, Tsourous G, Tzavara C, Georgiopoulos D, Katsanou P, Marakomichelakis G . Metabolic syndrome and incident cardiovascular morbidity and mortality in a Mediterranean hypertensive population. Am J Hypertens. 2007; 20(5):558-64. DOI: 10.1016/j.amjhyper.2006.12.001. View

Bales C, Buhr G . Is obesity bad for older persons? A systematic review of the pros and cons of weight reduction in later life. J Am Med Dir Assoc. 2008; 9(5):302-12. DOI: 10.1016/j.jamda.2008.01.006. View

Ravussin E, Lillioja S, Anderson T, Christin L, Bogardus C . Determinants of 24-hour energy expenditure in man. Methods and results using a respiratory chamber. J Clin Invest. 1986; 78(6):1568-78. PMC: 423919. DOI: 10.1172/JCI112749. View

Kimball S, Jefferson L . Control of translation initiation through integration of signals generated by hormones, nutrients, and exercise. J Biol Chem. 2010; 285(38):29027-32. PMC: 2937931. DOI: 10.1074/jbc.R110.137208. View

Anthony J, Anthony T, Kimball S, Jefferson L . Signaling pathways involved in translational control of protein synthesis in skeletal muscle by leucine. J Nutr. 2001; 131(3):856S-860S. DOI: 10.1093/jn/131.3.856S. View

Suryawan A, Jeyapalan A, Orellana R, Wilson F, Nguyen H, Davis T . Leucine stimulates protein synthesis in skeletal muscle of neonatal pigs by enhancing mTORC1 activation. Am J Physiol Endocrinol Metab. 2008; 295(4):E868-75. PMC: 2575905. DOI: 10.1152/ajpendo.90314.2008. View

Azadbakht L, Izadi V, Surkan P, Esmaillzadeh A . Effect of a High Protein Weight Loss Diet on Weight, High-Sensitivity C-Reactive Protein, and Cardiovascular Risk among Overweight and Obese Women: A Parallel Clinical Trial. Int J Endocrinol. 2013; 2013:971724. PMC: 3748746. DOI: 10.1155/2013/971724. View

Burd N, Yang Y, Moore D, Tang J, Tarnopolsky M, Phillips S . Greater stimulation of myofibrillar protein synthesis with ingestion of whey protein isolate v. micellar casein at rest and after resistance exercise in elderly men. Br J Nutr. 2012; 108(6):958-62. DOI: 10.1017/S0007114511006271. View

10.

Dardevet D, Sornet C, Bayle G, Prugnaud J, Pouyet C, Grizard J . Postprandial stimulation of muscle protein synthesis in old rats can be restored by a leucine-supplemented meal. J Nutr. 2002; 132(1):95-100. DOI: 10.1093/jn/132.1.95. View

11.

Duan Y, Li F, Li Y, Tang Y, Kong X, Feng Z . The role of leucine and its metabolites in protein and energy metabolism. Amino Acids. 2015; 48(1):41-51. DOI: 10.1007/s00726-015-2067-1. View

12.

Ispoglou T, White H, Preston T, McElhone S, McKenna J, Hind K . Double-blind, placebo-controlled pilot trial of L-Leucine-enriched amino-acid mixtures on body composition and physical performance in men and women aged 65-75 years. Eur J Clin Nutr. 2015; 70(2):182-8. PMC: 4744242. DOI: 10.1038/ejcn.2015.91. View

13.

Verreijen A, Verlaan S, Engberink M, Swinkels S, Bosch J, Weijs P . A high whey protein-, leucine-, and vitamin D-enriched supplement preserves muscle mass during intentional weight loss in obese older adults: a double-blind randomized controlled trial. Am J Clin Nutr. 2015; 101(2):279-86. DOI: 10.3945/ajcn.114.090290. View

14.

Zhang Y, Guo K, LeBlanc R, Loh D, Schwartz G, Yu Y . Increasing dietary leucine intake reduces diet-induced obesity and improves glucose and cholesterol metabolism in mice via multimechanisms. Diabetes. 2007; 56(6):1647-54. DOI: 10.2337/db07-0123. View

15.

Liang C, Curry B, Brown P, Zemel M . Leucine Modulates Mitochondrial Biogenesis and SIRT1-AMPK Signaling in C2C12 Myotubes. J Nutr Metab. 2014; 2014:239750. PMC: 4220583. DOI: 10.1155/2014/239750. View

16.

Westerterp-Plantenga M, Lemmens S, Westerterp K . Dietary protein - its role in satiety, energetics, weight loss and health. Br J Nutr. 2012; 108 Suppl 2:S105-12. DOI: 10.1017/S0007114512002589. View

17.

Zhou Y, Jetton T, Goshorn S, Lynch C, She P . Transamination is required for {alpha}-ketoisocaproate but not leucine to stimulate insulin secretion. J Biol Chem. 2010; 285(44):33718-26. PMC: 2962470. DOI: 10.1074/jbc.M110.136846. View

18.

McAllan L, Cotter P, Roche H, Korpela R, Nilaweera K . Impact of leucine on energy balance. J Physiol Biochem. 2012; 69(1):155-63. DOI: 10.1007/s13105-012-0170-2. View

19.

Alberti K, Eckel R, Grundy S, Zimmet P, Cleeman J, Donato K . Harmonizing the metabolic syndrome: a joint interim statement of the International Diabetes Federation Task Force on Epidemiology and Prevention; National Heart, Lung, and Blood Institute; American Heart Association; World Heart Federation;.... Circulation. 2009; 120(16):1640-5. DOI: 10.1161/CIRCULATIONAHA.109.192644. View

20.

Saghaei M . Random allocation software for parallel group randomized trials. BMC Med Res Methodol. 2004; 4:26. PMC: 533876. DOI: 10.1186/1471-2288-4-26. View