A Region-resolved Proteomic Map of the Human Brain Enabled by High-throughput Proteomics

Overview

Journal EMBO J

Specialties Biotechnology
Molecular Biology

Date 2023 Nov 2

PMID 37916885

Authors

Johanna Tushaus

Amirhossein Sakhteman

Severin Lechner

Matthew The

Eike Mucha

Christoph Krisp

Jurgen Schlegel

Claire Delbridge

Bernhard Kuster

Affiliations

Soon will be listed here.

Abstract

Substantial efforts are underway to deepen our understanding of human brain morphology, structure, and function using high-resolution imaging as well as high-content molecular profiling technologies. The current work adds to these approaches by providing a comprehensive and quantitative protein expression map of 13 anatomically distinct brain regions covering more than 11,000 proteins. This was enabled by the optimization, characterization, and implementation of a high-sensitivity and high-throughput microflow liquid chromatography timsTOF tandem mass spectrometry system (LC-MS/MS) capable of analyzing more than 2,000 consecutive samples prepared from formalin-fixed paraffin embedded (FFPE) material. Analysis of this proteomic resource highlighted brain region-enriched protein expression patterns and functional protein classes, protein localization differences between brain regions and individual markers for specific areas. To facilitate access to and ease further mining of the data by the scientific community, all data can be explored online in a purpose-built R Shiny app (https://brain-region-atlas.proteomics.ls.tum.de).

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