The Suppression of the Differentiation of Adipocytes with is Regulated Through the Posttranslational Modifications of C/EBPβ

Overview

Journal Food Sci Nutr

Specialty Biotechnology

Date 2023 Oct 13

PMID 37831750

Authors

Touko Nakano

Yutaro Sasaki

Toshio Norikura

Yusuke Hosokawa

Mayu Kasano

Isao Matsui-Yuasa

Xuedan Huang

Yoshinori Kobayashi

Akiko Kojima-Yuasa

Affiliations

Soon will be listed here.

Abstract

Obesity is a major risk factor for various chronic diseases, especially lifestyle-related diseases. Therefore, finding a protective substance against obesity and elucidating its molecular mechanism is one of the most important problems for improving human health. In this study, we investigated the antiobesity effect of extract (MFE). The aim of the study was to examine the in vivo and in vitro effects of MFE on lipid synthesis. We examined the effect using an in vivo experimental system with obesity model mice and an in vitro experimental system with 3T3-L1 preadipocytes. We found that the treatment of MFE significantly suppressed the increase in body weight and adipose tissue weight and morphological changes in the liver and adipose tissue of the obesity model mice. In the in vitro experimental system, we revealed that MFE treatment suppressed the expression of transcription factors such as C/EBPα, C/EBPβ, and PPARγ, which are involved in the early differentiation of 3T3-L1 preadipocytes. As a result, the ability to synthesize triacylglycerol was suppressed. An interesting finding in this study was the clarification that MFE decreases the expression of C/EBPβ through post-translation modifications (PTMs), followed by the transcriptional suppression of PPAR???? and C/EBP????.

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