Genome Editing in Rice Using CRISPR/Cas12i3

Overview

Journal Plant Biotechnol J

Specialties Biology
Biotechnology

Date 2023 Oct 12

PMID 37822083

Authors

Ping Lv

Fei Su

Fangyuan Chen

Chunxue Yan

Dandan Xia

Hui Sun

Shanshan Li

Zhiqiang Duan

Changle Ma

Hui Zhang

Mugui Wang

Xiaomu Niu

Jian-Kang Zhu

Jinshan Zhang

Affiliations

Soon will be listed here.

Abstract

The CRISPR/Cas type V-I is a family of programmable nuclease systems that prefers a T-rich protospacer adjacent motif (PAM) and is guided by a short crRNA. In this study, the genome-editing application of Cas12i3, a type V-I family endonuclease, was characterized in rice. We developed a CRIPSR/Cas12i3-based Multiplex direct repeats (DR)-spacer Array Genome Editing (iMAGE) system that was efficient in editing various genes in rice. Interestingly, iMAGE produced chromosomal structural variations with a higher frequency than CRISPR/Cas9. In addition, we developed base editors using deactivated Cas12i3 and generated herbicide-resistant rice plants using the base editors. These CRIPSR/Cas12i3-based genome editing systems will facilitate precision molecular breeding in plants.

Citing Articles

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