MiR-1301-3p Promotes Invasion and Migration and EMT Progression in Esophageal Cancer by Downregulating NBL1 Expression

Overview

Journal Thorac Cancer

Specialties Oncology
Pulmonary Medicine

Date 2023 Sep 8

PMID 37680006

Authors

Jianting Du

Shuliang Zhang

Xiaodan Zhang

Zhang Yang

Songtao Xue

Guobing Xu

Bin Zheng

Chun Chen

Affiliations

Soon will be listed here.

Abstract

Background: Esophageal cancer (ESCA) is one of the most aggressive and lethal human malignant cancers. MicroRNA-1301-3p (miR-1301-3p) plays vital roles in a majority of malignancies. The aim of this study was to investigate the role of miR-1301-3p/NBL1 axis on ESCA cell invasion, migration, epithelial-mesenchymal transition (EMT) process, as well as its association with prognosis of ESCA patients.

Methods: The expression levels of miR-1301-3p and NBL1 were predicted by bioinformatics and further verified by RT-qPCR assays. Kaplan-Meier (K-M) plotter analysis and univariate and multivariate Cox analyses were used to evaluate the relationship between miR-1301-3p and clinicopathological variables and prognosis. The role of miR-1301-3p on cell invasion, migration was detected by transwell invasion, and wound healing assays, respectively. The EMT-related proteins were detected by western blot. The target genes and the target binding sites were predicted by bioinformatics and further determined by RT-qPCR assay.

Results: MiR-1301-3p was remarkably upregulated in ESCA tissues and cells, and its high expression was associated with poor prognosis of ESCA. Overexpression of miR-1301-3p promoted ESCA cell invasion, migration and mediated EMT process in vitro, whereas knockdown of miR-1301-3p showed the opposite effects. Moreover, NBL1 was predicted as a target gene of miR-1301-3p. NBL1 was lowly expressed in ESCA cells and significantly decreased after upregulation of miR-1301-3p. Meanwhile, we found that low expression of NBL1 was significantly associated with poor prognosis of ESCA patients.

Conclusion: MiR-1301-3p is a potential biomarker for predicting the prognosis of ESCA patients. It may promote ESCA invasion, migration and EMT progression by regulating NBL1 expression.

Citing Articles

Association between cancer-associated fibroblasts and prognosis of neoadjuvant chemoradiotherapy in esophageal squamous cell carcinoma: a bioinformatics analysis based on single-cell RNA sequencing.

Huang Z, Cong Z, Luo J, Qiu B, Wang K, Gao C Cancer Cell Int. 2025; 25(1):74.

PMID: 40025479 PMC: 11871762. DOI: 10.1186/s12935-025-03709-x.

miR-1301-3p promotes invasion and migration and EMT progression in esophageal cancer by downregulating NBL1 expression.

Du J, Zhang S, Zhang X, Yang Z, Xue S, Xu G Thorac Cancer. 2023; 14(30):3032-3041.

PMID: 37680006 PMC: 10599971. DOI: 10.1111/1759-7714.15093.

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