Structural and Functional Characterization of Aedes Aegypti Pupal Cuticle Protein That Controls Dengue Virus Infection

Overview

Journal Protein Sci

Specialty Biochemistry

Date 2023 Aug 18

PMID 37593853

Authors

Qingqing Huang

Edem Gavor

Nikhil Kumar Tulsian

Jingsong Fan

Qingsong Lin

Yu Keung Mok

R Manjunatha Kini

J Sivaraman

Affiliations

Soon will be listed here.

Abstract

The pupal cuticle protein from Aedes aegypti (AaPC) inhibits dengue virus (DENV) infection; however, the underlying mechanism of this inhibition remains unknown. Here, we report that AaPC is an intrinsically disordered protein and interacts with domain I/II of the DENV envelope protein via residues Asp59, Asp61, Glu71, Asp73, Ser75, and Asp80. AaPC can directly bind to and cause the aggregation of DENV, which in turn blocks virus infection during the virus-cell fusion stage. AaPC may also influence viral recognition and attachment by interacting with human immune receptors DC-SIGN and CD4. These findings enhance our understanding of the role of AaPC in mitigating viral infection and suggest that AaPC is a potential target for developing inhibitors or antibodies to control dengue virus infection.

Citing Articles

Structural and functional characterization of Aedes aegypti pupal cuticle protein that controls dengue virus infection.

Huang Q, Gavor E, Tulsian N, Fan J, Lin Q, Mok Y Protein Sci. 2023; 32(10):e4761.

PMID: 37593853 PMC: 10510476. DOI: 10.1002/pro.4761.

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