Development of Reproductive Engineering Techniques at the RIKEN BioResource Center

Overview

Journal Exp Anim

Date 2016 Oct 21

PMID 27760894

Citations 3

Authors

Atsuo Ogura

Affiliations

Soon will be listed here.

Abstract

Reproductive engineering techniques are essential for assisted reproduction of animals and generation of genetically modified animals. They may also provide invaluable research models for understanding the mechanisms involved in the developmental and reproductive processes. At the RIKEN BioResource Center (BRC), I have sought to develop new reproductive engineering techniques, especially those related to cryopreservation, microinsemination (sperm injection), nuclear transfer, and generation of new stem cell lines and animals, hoping that they will support the present and future projects at BRC. I also want to combine our techniques with genetic and biochemical analyses to solve important biological questions. We expect that this strategy makes our research more unique and refined by providing deeper insights into the mechanisms that govern the reproductive and developmental systems in mammals. To make this strategy more effective, it is critical to work with experts in different scientific fields. I have enjoyed collaborations with about 100 world-recognized laboratories, and all our collaborations have been successful and fruitful. This review summarizes development of reproductive engineering techniques at BRC during these 15 years.

Citing Articles

Comparison of the effect of two commercialized vitrification carriers on pregnancy outcomes in freeze-thaw cycles.

Zhu H, Li L, Zhang H, Jiang Y, Liu R, Xi Q J Int Med Res. 2023; 51(8):3000605231187948.

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Improvement of short straws for sperm cryopreservation: installing an air-permeable filter facilitates handling.

Kaneko R, Kakinuma T, Sato S, Jinno-Oue A J Reprod Dev. 2021; 67(3):235-239.

PMID: 33853994 PMC: 8238672. DOI: 10.1262/jrd.2021-019.

Freezing sperm in short straws reduces storage space and allows transport in dry ice.

Kaneko R, Kakinuma T, Sato S, Jinno-Oue A J Reprod Dev. 2018; 64(6):541-545.

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References

Inoue K, Ogonuki N, Miki H, Hirose M, Noda S, Kim J . Inefficient reprogramming of the hematopoietic stem cell genome following nuclear transfer. J Cell Sci. 2006; 119(Pt 10):1985-91. DOI: 10.1242/jcs.02913. View

Honda A, Hatori M, Hirose M, Honda C, Izu H, Inoue K . Naive-like conversion overcomes the limited differentiation capacity of induced pluripotent stem cells. J Biol Chem. 2013; 288(36):26157-26166. PMC: 3764818. DOI: 10.1074/jbc.M113.502492. View

Ogura A, Suzuki O, Tanemura K, Mochida K, Kobayashi Y, Matsuda J . Development of normal mice from metaphase I oocytes fertilized with primary spermatocytes. Proc Natl Acad Sci U S A. 1998; 95(10):5611-5. PMC: 20426. DOI: 10.1073/pnas.95.10.5611. View

Matoba S, Ogura A . Generation of functional oocytes and spermatids from fetal primordial germ cells after ectopic transplantation in adult mice. Biol Reprod. 2010; 84(4):631-8. DOI: 10.1095/biolreprod.110.087122. View

Fulka Jr J, Miyashita N, Nagai T, Ogura A . Do cloned mammals skip a reprogramming step?. Nat Biotechnol. 2004; 22(1):25-6. DOI: 10.1038/nbt0104-25. View

Kohama C, Kato H, Numata K, Hirose M, Takemasa T, Ogura A . ES cell differentiation system recapitulates the establishment of imprinted gene expression in a cell-type-specific manner. Hum Mol Genet. 2011; 21(6):1391-401. DOI: 10.1093/hmg/ddr577. View

Honda A, Hirose M, Hatori M, Matoba S, Miyoshi H, Inoue K . Generation of induced pluripotent stem cells in rabbits: potential experimental models for human regenerative medicine. J Biol Chem. 2010; 285(41):31362-9. PMC: 2951210. DOI: 10.1074/jbc.M110.150540. View

Hasegawa A, Yonezawa K, Ohta A, Mochida K, Ogura A . Optimization of a protocol for cryopreservation of mouse spermatozoa using cryotubes. J Reprod Dev. 2011; 58(1):156-61. DOI: 10.1262/jrd.11-097n. View

Yokonishi T, Sato T, Komeya M, Katagiri K, Kubota Y, Nakabayashi K . Offspring production with sperm grown in vitro from cryopreserved testis tissues. Nat Commun. 2014; 5:4320. DOI: 10.1038/ncomms5320. View

10.

Nagai A, Hattori T, Hirose M, Ogura A, Nozaki K, Aizawa M . Mouse embryonic stem cells cultured under serum- and feeder-free conditions maintain their self-renewal capacity on hydroxyapatite. Mater Sci Eng C Mater Biol Appl. 2013; 34:214-20. DOI: 10.1016/j.msec.2013.09.012. View

11.

Ogonuki N, Inoue K, Ogura A . Birth of normal mice following round spermatid injection without artificial oocyte activation. J Reprod Dev. 2011; 57(4):534-8. DOI: 10.1262/jrd.11-008m. View

12.

Kanatsu-Shinohara M, Ogonuki N, Inoue K, Miki H, Ogura A, Toyokuni S . Long-term proliferation in culture and germline transmission of mouse male germline stem cells. Biol Reprod. 2003; 69(2):612-6. DOI: 10.1095/biolreprod.103.017012. View

13.

Kushiyama A, Sakoda H, Oue N, Okubo M, Nakatsu Y, Ono H . Resistin-like molecule β is abundantly expressed in foam cells and is involved in atherosclerosis development. Arterioscler Thromb Vasc Biol. 2013; 33(8):1986-93. DOI: 10.1161/ATVBAHA.113.301546. View

14.

Ogonuki N, Inoue K, Miki H, Mochida K, Hatori M, Okada H . Differential development of rabbit embryos following microinsemination with sperm and spermatids. Mol Reprod Dev. 2005; 72(3):411-7. DOI: 10.1002/mrd.20363. View

15.

Sanli I, Feil R . Chromatin mechanisms in the developmental control of imprinted gene expression. Int J Biochem Cell Biol. 2015; 67:139-47. DOI: 10.1016/j.biocel.2015.04.004. View

16.

Oikawa M, Inoue K, Shiura H, Matoba S, Kamimura S, Hirose M . Understanding the X chromosome inactivation cycle in mice: a comprehensive view provided by nuclear transfer. Epigenetics. 2013; 9(2):204-11. PMC: 3962530. DOI: 10.4161/epi.26939. View

17.

Ogura A, Ogonuki N, Miki H, Inoue K . Microinsemination and nuclear transfer using male germ cells. Int Rev Cytol. 2005; 246:189-229. DOI: 10.1016/S0074-7696(05)46005-2. View

18.

Honda A, Hirose M, Ogura A . Basic FGF and Activin/Nodal but not LIF signaling sustain undifferentiated status of rabbit embryonic stem cells. Exp Cell Res. 2009; 315(12):2033-42. DOI: 10.1016/j.yexcr.2009.01.024. View

19.

Honda A, Hirose M, Inoue K, Ogonuki N, Miki H, Shimozawa N . Stable embryonic stem cell lines in rabbits: potential small animal models for human research. Reprod Biomed Online. 2008; 17(5):706-15. DOI: 10.1016/s1472-6483(10)60320-3. View

20.

Sugimoto M, Kondo M, Hirose M, Suzuki M, Mekada K, Abe T . Molecular identification of t(w5): Vps52 promotes pluripotential cell differentiation through cell-cell interactions. Cell Rep. 2012; 2(5):1363-74. DOI: 10.1016/j.celrep.2012.10.004. View