Transcription-Replication Conflicts As a Source of Genome Instability

Overview

Journal Annu Rev Genet

Publisher Annual Reviews

Specialty Genetics

Date 2023 Aug 8

PMID 37552891

Authors

Liana Goehring

Tony T Huang

Duncan J Smith

Affiliations

Soon will be listed here.

Abstract

Transcription and replication both require large macromolecular complexes to act on a DNA template, yet these machineries cannot simultaneously act on the same DNA sequence. Conflicts between the replication and transcription machineries (transcription-replication conflicts, or TRCs) are widespread in both prokaryotes and eukaryotes and have the capacity to both cause DNA damage and compromise complete, faithful replication of the genome. This review will highlight recent studies investigating the genomic locations of TRCs and the mechanisms by which they may be prevented, mitigated, or resolved. We address work from both model organisms and mammalian systems but predominantly focus on multicellular eukaryotes owing to the additional complexities inherent in the coordination of replication and transcription in the context of cell type-specific gene expression and higher-order chromatin organization.

Citing Articles

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Transcription near arrested DNA replication forks triggers ribosomal DNA copy number changes.

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Histone variant H2A.Z is needed for efficient transcription-coupled NER and genome integrity in UV challenged yeast cells.

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RAD52 resolves transcription-replication conflicts to mitigate R-loop induced genome instability.

Jalan M, Sharma A, Pei X, Weinhold N, Buechelmaier E, Zhu Y Nat Commun. 2024; 15(1):7776.

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