Cytoplasmically Localized TRNA-derived Fragments Inhibit Translation in S2 Cells

Overview

Journal Turk J Biol

Specialty Biology

Date 2023 Aug 2

PMID 37529257

Authors

Syed Muhammad Hamid

Bunyamin Akgul

Affiliations

Soon will be listed here.

Abstract

Transfer ribonucleic acids (tRNAs) serve not only as amino acid carriers during translation but also as a template for the biogenesis of short fragments that can regulate gene expression. Despite recent progress in the function of tRNA-derived fragments (tRFs), their intracellular localization, protein partners, and role in regulating translation are not well understood. We used synthetic tRFs to investigate their localization and function in S2 cells. Under our experimental setting, all synthetic tRFs tested were localized at distinct sites within the cytoplasm in a similar manner in S2 cells. Cytoplasmically-localized tRFs were positioned in close proximity to GW182 and XRN1 proteins. Functionally, tRFs, which slightly suppressed proliferation in S2 cells, inhibited translation without any major shift in the polysome profile. These results suggest that 5'-tRFs are cytoplasmically-localized and regulate gene expression through inhibition of translation in .

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