Intracellular Growth of Brucella is Mediated by Dps-dependent Activation of Ferritinophagy

Overview

Journal EMBO Rep

Specialty Molecular Biology

Date 2023 Jul 28

PMID 37503678

Authors

Huynh Tan Hop

Tran Xuan Ngoc Huy

Hu Jang Lee

Suk Kim

Affiliations

Soon will be listed here.

Abstract

Bacteria of the genus Brucella cause brucellosis, one of the world's most common zoonotic diseases. A major contributor to Brucella's virulence is the ability to circumvent host immune defense mechanisms. Here, we find that the DNA-binding protein Dps from Brucella is secreted within the macrophage cytosol, modulating host iron homeostasis and mediating intracellular growth of Brucella. In addition to dampening iron-dependent production of reactive oxygen species (ROS), a key immune effector required for immediate bacterial clearance, cytosolic Dps mediates ferritinophagy activation to elevate intracellular free-iron levels, thereby promoting Brucella growth and inducing host cell necrosis. Inactivation of the ferritinophagy pathway by Ncoa4 gene knockout significantly inhibits intracellular growth of Brucella and host cell death. Our study uncovers an unconventional role of bacterial Dps, identifying a crucial virulence mechanism used by Brucella to adapt to the harsh environment inside macrophages.

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Intracellular growth of Brucella is mediated by Dps-dependent activation of ferritinophagy.

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