Dual-color DNA-PAINT Single-particle Tracking Enables Extended Studies of Membrane Protein Interactions

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Jul 19

PMID 37468504

Authors

Christian Niederauer

Chikim Nguyen

Miles Wang-Henderson

Johannes Stein

Sebastian Strauss

Alexander Cumberworth

Florian Stehr

Ralf Jungmann

Petra Schwille

Kristina A Ganzinger

Affiliations

Soon will be listed here.

Abstract

DNA-PAINT based single-particle tracking (DNA-PAINT-SPT) has recently significantly enhanced observation times in in vitro SPT experiments by overcoming the constraints of fluorophore photobleaching. However, with the reported implementation, only a single target can be imaged and the technique cannot be applied straight to live cell imaging. Here we report on leveraging this technique from a proof-of-principle implementation to a useful tool for the SPT community by introducing simultaneous live cell dual-color DNA-PAINT-SPT for quantifying protein dimerization and tracking proteins in living cell membranes, demonstrating its improved performance over single-dye SPT.

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Dual-color DNA-PAINT single-particle tracking enables extended studies of membrane protein interactions.

Niederauer C, Nguyen C, Wang-Henderson M, Stein J, Strauss S, Cumberworth A Nat Commun. 2023; 14(1):4345.

PMID: 37468504 PMC: 10356854. DOI: 10.1038/s41467-023-40065-8.

The K2: Open-source simultaneous triple-color TIRF microscope for live-cell and single-molecule imaging.

Niederauer C, Seynen M, Zomerdijk J, Kamp M, Ganzinger K HardwareX. 2023; 13:e00404.

PMID: 36923558 PMC: 10009532. DOI: 10.1016/j.ohx.2023.e00404.

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