Visualization of Small Intact Proteins in Breast Cancer FFPE Tissue

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Jul 6

PMID 37410292

Authors

Marco Giampa

Maria K Andersen

Sebastian Krossa

Vanna Denti

Andrew Smith

Siver Andreas Moestue

Affiliations

Soon will be listed here.

Abstract

Molecular visualization of metabolites, lipids, and proteins by matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) is becoming an in-demand analytical approach to aid the histopathological analysis of breast cancer. Particularly, proteins seem to play a role in cancer progression, and specific proteins are currently used in the clinic for staging. Formalin-fixed paraffin-embedded (FFPE) tissues are ideal for correlating the molecular markers with clinical outcomes due to their long-term storage. So far, to obtain proteomic information by MSI from this kind of tissue, antigen retrieval and tryptic digestion steps are required. In this chapter, we present a protocol to spatially detect small proteins in tumor and necrotic regions of patient-derived breast cancer xenograft FFPE tissues without employing any on-tissue digestion. This protocol can be used for other kinds of FFPE tissue following specific optimization of the sample preparation phases.

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