Direct Mapping of Kidney Function by DCE-MRI Urography Using a Tetrazinanone Organic Radical Contrast Agent

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Jul 5

PMID 37407664

Authors

Nicholas D Calvert

Alexia Kirby

Mojmir Suchy

Peter Pallister

Aidan A Torrens

Dylan Burger

Gerd Melkus

Nicola Schieda

Adam J Shuhendler

Affiliations

Soon will be listed here.

Abstract

Chronic kidney disease (CKD) and acute kidney injury (AKI) are ongoing global health burdens. Glomerular filtration rate (GFR) is the gold standard measure of kidney function, with clinical estimates providing a global assessment of kidney health without spatial information of kidney- or region-specific dysfunction. The addition of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) to the anatomical imaging already performed would yield a 'one-stop-shop' for renal assessment in cases of suspected AKI and CKD. Towards urography by DCE-MRI, we evaluated a class of nitrogen-centered organic radicals known as verdazyls, which are extremely stable even in highly reducing environments. A glucose-modified verdazyl, glucoverdazyl, provided contrast limited to kidney and bladder, affording functional kidney evaluation in mouse models of unilateral ureteral obstruction (UUO) and folic acid-induced nephropathy (FAN). Imaging outcomes correlated with histology and hematology assessing kidney dysfunction, and glucoverdazyl clearance rates were found to be a reliable surrogate measure of GFR.

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