Mycoprotein Production by Submerged Fermentation of the Edible Mushroom in a Batch Stirred Tank Bioreactor Using Agro-Industrial Hydrolysate

Overview

Journal Foods

Specialty Biotechnology

Date 2023 Jun 28

PMID 37372506

Authors

Georgios Bakratsas

Angeliki Polydera

Oskar Nilson

Alexandra V Chatzikonstantinou

Charilaos Xiros

Petros Katapodis

Haralambos Stamatis

Affiliations

Soon will be listed here.

Abstract

The demand for cheap, healthy, and sustainable alternative protein sources has turned research interest into microbial proteins. Mycoproteins prevail due to their quite balanced amino acid profile, low carbon footprint and high sustainability potential. The goal of this research was to investigate the capability of to metabolize the main sugars of agro-industrial side streams, such as aspen wood chips hydrolysate, to produce high-value protein with low cost. Our results indicate that LGAM 1123 could be cultivated both in a C-6 (glucose)- and C-5(xylose)-sugar-containing medium for mycoprotein production. A mixture of glucose and xylose was found to be ideal for biomass production with high protein content and rich amino acid profile. LGAM 1123 cultivation in a 4 L stirred-tank bioreactor using aspen hydrolysate was achieved with 25.0 ± 3.4 g L biomass production, 1.8 ± 0.4 d specific growth rate and a protein yield of 54.5 ± 0.5% (g/100 g sugars). PCA analysis of the amino acids revealed a strong correlation between the amino acid composition of the protein produced and the ratios of glucose and xylose in the culture medium. The production of high-nutrient mycoprotein by submerged fermentation of the edible fungus using agro-industrial hydrolysates is a promising bioprocess in the food and feed industry.

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References

Wohlbach D, Kuo A, Sato T, Potts K, Salamov A, LaButti K . Comparative genomics of xylose-fermenting fungi for enhanced biofuel production. Proc Natl Acad Sci U S A. 2011; 108(32):13212-7. PMC: 3156214. DOI: 10.1073/pnas.1103039108. View

Papaspyridi L, Aligiannis N, Topakas E, Christakopoulos P, Skaltsounis A, Fokialakis N . Submerged fermentation of the edible mushroom Pleurotus ostreatus in a batch stirred tank bioreactor as a promising alternative for the effective production of bioactive metabolites. Molecules. 2012; 17(3):2714-24. PMC: 6268715. DOI: 10.3390/molecules17032714. View

Gardeli C, Athenaki M, Xenopoulos E, Mallouchos A, Koutinas A, Aggelis G . Lipid production and characterization by Mortierella (Umbelopsis) isabellina cultivated on lignocellulosic sugars. J Appl Microbiol. 2017; 123(6):1461-1477. DOI: 10.1111/jam.13587. View

Souza Filho P, Nair R, Andersson D, Lennartsson P, Taherzadeh M . Vegan-mycoprotein concentrate from pea-processing industry byproduct using edible filamentous fungi. Fungal Biol Biotechnol. 2018; 5:5. PMC: 5880086. DOI: 10.1186/s40694-018-0050-9. View

Sydor M, Cofta G, Doczekalska B, Bonenberg A . Fungi in Mycelium-Based Composites: Usage and Recommendations. Materials (Basel). 2022; 15(18). PMC: 9505859. DOI: 10.3390/ma15186283. View

Kim K, Choi B, Lee I, Lee H, Kwon S, Oh K . Bioproduction of mushroom mycelium of Agaricus bisporus by commercial submerged fermentation for the production of meat analogue. J Sci Food Agric. 2011; 91(9):1561-8. DOI: 10.1002/jsfa.4348. View

Xiao Q, Ma F, Li Y, Yu H, Li C, Zhang X . Differential Proteomic Profiles of in Response to Lignocellulosic Components Provide Insights into Divergent Adaptive Mechanisms. Front Microbiol. 2017; 8:480. PMC: 5362632. DOI: 10.3389/fmicb.2017.00480. View

Lauria A, Ippolito M, Almerico A . Principal component analysis on molecular descriptors as an alternative point of view in the search of new Hsp90 inhibitors. Comput Biol Chem. 2009; 33(5):386-90. DOI: 10.1016/j.compbiolchem.2009.07.010. View

Cohen N, Cohen J, Asatiani M, Varshney V, Yu H, Yang Y . Chemical composition and nutritional and medicinal value of fruit bodies and submerged cultured mycelia of culinary-medicinal higher Basidiomycetes mushrooms. Int J Med Mushrooms. 2014; 16(3):273-91. DOI: 10.1615/intjmedmushr.v16.i3.80. View

10.

Gern R, Wisbeck E, Rampinelli J, Ninow J, Furlan S . Alternative medium for production of Pleurotus ostreatus biomass and potential antitumor polysaccharides. Bioresour Technol. 2007; 99(1):76-82. DOI: 10.1016/j.biortech.2006.11.059. View

11.

Smiderle F, Olsen L, Ruthes A, Czelusniak P, Santana-Filho A, Sassaki G . Exopolysaccharides, proteins and lipids in Pleurotus pulmonarius submerged culture using different carbon sources. Carbohydr Polym. 2021; 87(1):368-376. DOI: 10.1016/j.carbpol.2011.07.063. View

12.

Wordofa G, Kristensen M . Tolerance and metabolic response of VLB120 towards biomass hydrolysate-derived inhibitors. Biotechnol Biofuels. 2018; 11:199. PMC: 6052574. DOI: 10.1186/s13068-018-1192-y. View

13.

Koppram R, Tomas-Pejo E, Xiros C, Olsson L . Lignocellulosic ethanol production at high-gravity: challenges and perspectives. Trends Biotechnol. 2013; 32(1):46-53. DOI: 10.1016/j.tibtech.2013.10.003. View

14.

Liu Q, Zhou P, Luo P, Wu P . Occurrence of Furfural and Its Derivatives in Coffee Products in China and Estimation of Dietary Intake. Foods. 2023; 12(1). PMC: 9818524. DOI: 10.3390/foods12010200. View

15.

Jagtap S, Dhiman S, Kim T, Li J, Lee J, Kang Y . Enzymatic hydrolysis of aspen biomass into fermentable sugars by using lignocellulases from Armillaria gemina. Bioresour Technol. 2013; 133:307-14. DOI: 10.1016/j.biortech.2013.01.118. View

16.

Liu D, Zhang Y, Li J, Sun W, Yao Y, Tian C . The Weimberg pathway: an alternative for Myceliophthora thermophila to utilize D-xylose. Biotechnol Biofuels Bioprod. 2023; 16(1):13. PMC: 9869559. DOI: 10.1186/s13068-023-02266-7. View

17.

Elisashvili V . Submerged cultivation of medicinal mushrooms: bioprocesses and products (review). Int J Med Mushrooms. 2012; 14(3):211-39. DOI: 10.1615/intjmedmushr.v14.i3.10. View

18.

Pellegrino R, Blasi F, Angelini P, Ianni F, Alabed H, Emiliani C . LC/MS Q-TOF Metabolomic Investigation of Amino Acids and Dipeptides in Grown on Different Substrates. J Agric Food Chem. 2022; 70(33):10371-10382. PMC: 9413224. DOI: 10.1021/acs.jafc.2c04197. View

19.

Hou J, Suo F, Wang C, Li X, Shen Y, Bao X . Fine-tuning of NADH oxidase decreases byproduct accumulation in respiration deficient xylose metabolic Saccharomyces cerevisiae. BMC Biotechnol. 2014; 14:13. PMC: 3928090. DOI: 10.1186/1472-6750-14-13. View

20.

Zhao Z, Xian M, Liu M, Zhao G . Biochemical routes for uptake and conversion of xylose by microorganisms. Biotechnol Biofuels. 2020; 13:21. PMC: 6995148. DOI: 10.1186/s13068-020-1662-x. View