In Vitro and in Vivo Inhibitory Activity of C-glycoside Flavonoid Extracts from Mung Bean Coat on Pancreatic Lipase and α-glucosidase

Overview

Journal Plant Foods Hum Nutr

Specialties Biology
Nutritional Sciences

Date 2023 Jun 23

PMID 37351712

Authors

Jin-Cang Ruan

Rui-Yan Peng

Yi-Ting Chen

Hai-Xia Xu

Qing-Feng F Zhang

Affiliations

Soon will be listed here.

Abstract

Mung bean is a kind of legume commonly eaten by human. In the present study, a HPLC method for analyzing of two C-glycoside flavonoids, isovitexin and vitexin, in Mung bean was developed. Results showed that the flavonoids are mainly existed in Mung bean coat (MBC), while kernel contains very trace. The extraction of C-glycoside flavonoids from MBC was optimized. MBC extracts with isovitexin and vitexin contents of 29.0 ± 0.28% and 35.8 ± 0.19% were obtained with yield of 1.6 ± 0.21%. MBC extracts exhibited inhibitory activities on pancreatic lipase and α-glucosidase with IC values of 0.147 mg/ml and 0.226 mg/ml, respectively. The inhibitory kinetics revealed that MBC extracts showed mixed-type inhibition on these enzymes. Fluorescence quenching titration confirmed the binding of MBC extracts with the enzyme proteins. In vivo study revealed that pre-administration with MBC extracts significantly reduced the triglyceride absorption. Furthermore, it also improved postprandial hyperglycemia in rats through the inhibition of α-glucosidase.

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References

Liu T, Liu X, Chen Q, Shi Y . Lipase Inhibitors for Obesity: A Review. Biomed Pharmacother. 2020; 128:110314. DOI: 10.1016/j.biopha.2020.110314. View

Maguire D, Talwar D, Shiels P, McMillan D . The role of thiamine dependent enzymes in obesity and obesity related chronic disease states: A systematic review. Clin Nutr ESPEN. 2018; 25:8-17. DOI: 10.1016/j.clnesp.2018.02.007. View

Etxeberria U, De la Garza A, Campion J, Martinez J, Milagro F . Antidiabetic effects of natural plant extracts via inhibition of carbohydrate hydrolysis enzymes with emphasis on pancreatic alpha amylase. Expert Opin Ther Targets. 2012; 16(3):269-97. DOI: 10.1517/14728222.2012.664134. View

Feng J, Yang X, Wang R . Bio-assay guided isolation and identification of α-glucosidase inhibitors from the leaves of Aquilaria sinensis. Phytochemistry. 2011; 72(2-3):242-7. DOI: 10.1016/j.phytochem.2010.11.025. View

Fatmawati S, Shimizu K, Kondo R . Ganoderol B: a potent α-glucosidase inhibitor isolated from the fruiting body of Ganoderma lucidum. Phytomedicine. 2011; 18(12):1053-5. DOI: 10.1016/j.phymed.2011.03.011. View

Dahiya P, Linnemann A, van Boekel M, Khetarpaul N, Grewal R, Nout M . Mung bean: technological and nutritional potential. Crit Rev Food Sci Nutr. 2014; 55(5):670-88. DOI: 10.1080/10408398.2012.671202. View

Xu H, Zhou Q, Liu B, Cheng K, Chen F, Wang M . Neuroprotective Potential of Mung Bean ( L.) Polyphenols in Alzheimer's Disease: A Review. J Agric Food Chem. 2021; 69(39):11554-11571. DOI: 10.1021/acs.jafc.1c04049. View

Hou D, Yousaf L, Xue Y, Hu J, Wu J, Hu X . Mung Bean ( L.): Bioactive Polyphenols, Polysaccharides, Peptides, and Health Benefits. Nutrients. 2019; 11(6). PMC: 6627095. DOI: 10.3390/nu11061238. View

Luo J, Cai W, Wu T, Xu B . Phytochemical distribution in hull and cotyledon of adzuki bean (Vigna angularis L.) and mung bean (Vigna radiate L.), and their contribution to antioxidant, anti-inflammatory and anti-diabetic activities. Food Chem. 2016; 201:350-60. DOI: 10.1016/j.foodchem.2016.01.101. View

10.

Peng X, Zhang G, Liao Y, Gong D . Inhibitory kinetics and mechanism of kaempferol on α-glucosidase. Food Chem. 2015; 190:207-215. DOI: 10.1016/j.foodchem.2015.05.088. View

11.

Nasri R, Bidel L, Rugani N, Perrier V, Carriere F, Dubreucq E . Inhibition of CpLIP2 Lipase Hydrolytic Activity by Four Flavonols (Galangin, Kaempferol, Quercetin, Myricetin) Compared to Orlistat and Their Binding Mechanisms Studied by Quenching of Fluorescence. Molecules. 2019; 24(16). PMC: 6719172. DOI: 10.3390/molecules24162888. View

12.

Basque J, Menard D . Establishment of culture systems of human gastric epithelium for the study of pepsinogen and gastric lipase synthesis and secretion. Microsc Res Tech. 2000; 48(5):293-302. DOI: 10.1002/(SICI)1097-0029(20000301)48:5<293::AID-JEMT6>3.0.CO;2-A. View

13.

Aloulou A, Carriere F . Gastric lipase: an extremophilic interfacial enzyme with medical applications. Cell Mol Life Sci. 2008; 65(6):851-4. PMC: 11131824. DOI: 10.1007/s00018-008-7546-z. View

14.

Nair A, Jacob S . A simple practice guide for dose conversion between animals and human. J Basic Clin Pharm. 2016; 7(2):27-31. PMC: 4804402. DOI: 10.4103/0976-0105.177703. View