Transcriptional Space-time Mapping Identifies Concerted Immune and Stromal Cell Patterns and Gene Programs in Wound Healing and Cancer

Overview

Journal Cell Stem Cell

Publisher Cell Press

Specialty Cell Biology

Date 2023 Jun 2

PMID 37267918

Authors

Kenneth H Hu

Nicholas F Kuhn

Tristan Courau

Jessica Tsui

Bushra Samad

Patrick Ha

Johannes R Kratz

Alexis J Combes

Matthew F Krummel

Affiliations

Soon will be listed here.

Abstract

Tissue repair responses in metazoans are highly coordinated by different cell types over space and time. However, comprehensive single-cell-based characterization covering this coordination is lacking. Here, we captured transcriptional states of single cells over space and time during skin wound closure, revealing choreographed gene-expression profiles. We identified shared space-time patterns of cellular and gene program enrichment, which we call multicellular "movements" spanning multiple cell types. We validated some of the discovered space-time movements using large-volume imaging of cleared wounds and demonstrated the value of this analysis to predict "sender" and "receiver" gene programs in macrophages and fibroblasts. Finally, we tested the hypothesis that tumors are like "wounds that never heal" and found conserved wound healing movements in mouse melanoma and colorectal tumor models, as well as human tumor samples, revealing fundamental multicellular units of tissue biology for integrative studies.

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