Small RNA Profiling of Aster Yellows Phytoplasma-Infected Plants Showing Different Symptoms

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2023 May 27

PMID 37239473

Authors

Nicoletta Contaldo

Yuri Zambon

Zsuszanna Nagyne Galbacs

Fabio Miloro

Zoltan Havelda

Assunta Bertaccini

Eva Varallyay

Affiliations

Soon will be listed here.

Abstract

Micropropagated plants infected with ' Phytoplasma asteris' showed virescence symptoms, witches' broom symptoms, or became asymptomatic after their planting in pots. Nine plants were grouped into three categories according to these symptoms, which were then employed for investigation. The phytoplasma concentration, as determined by qPCR, correlated well with the severity of symptoms. To reveal the changes in the small RNA profiles in these plants, small RNA high-throughput sequencing (HTS) was carried out. The bioinformatics comparison of the micro (mi) RNA and small interfering (si) RNA profiles of the symptomatic and asymptomatic plants showed changes, which could be correlated to some of the observed symptoms. These results complement previous studies on phytoplasmas and serve as a starting point for small RNA-omic studies in phytoplasma research.

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