A Novel AP2/ERF Transcription Factor CR1 Regulates the Accumulation of Vindoline and Serpentine in

Overview

Journal Front Plant Sci

Date 2017 Dec 23

PMID 29270185

Citations 22

Authors

Jiaqi Liu

Fangyuan Gao

Juansheng Ren

Xianjun Lu

Guangjun Ren

Rui Wang

Affiliations

Soon will be listed here.

Abstract

As one type of the most important alkaloids in the world, terpenoid indole alkaloids (TIAs) show a wide range of pharmaceutical activities that are beneficial for clinical treatments. produces approximately 130 identified TIAs and is considered to be a model plant to study TIA biosynthesis. In order to increase the production of high medical value metabolites whose yields are extremely low in , genetic engineering combined with transcriptional regulation has been applied in recent years. By using bioinformatics which is based on RNA sequencing (RNA-seq) data from methyl jasmonate (MeJA)-treated as well as phylogenetic analysis, the present work aims to screen candidate genes that may be involved in the regulation of TIA biosynthesis, resulting in a novel AP2/ERF transcription factor, CR1 (Catharanthus roseus 1). Subsequently, virus-induced gene silencing (VIGS) of was carried out to identify the involvement of CR1 in the accumulations of several TIAs and quantitative real-time PCR (qRT-PCR) was then applied to detect the expression levels of 7 genes in the related biosynthetic pathway in silenced plants. The results show that all the 7 genes were upregulated in -silenced plants. Furthermore, metabolite analyses indicate that silencing could increase the accumulations of vindoline and serpentine in . These results suggest a novel negative regulator which may be involved in the TIAs biosynthetic pathway.

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References

Meng D, Yu X, Ma L, Hu J, Liang Y, Liu X . Transcriptomic Response of Chinese Yew () to Cold Stress. Front Plant Sci. 2017; 8:468. PMC: 5408010. DOI: 10.3389/fpls.2017.00468. View

Montiel G, Zarei A, Korbes A, Memelink J . The jasmonate-responsive element from the ORCA3 promoter from Catharanthus roseus is active in Arabidopsis and is controlled by the transcription factor AtMYC2. Plant Cell Physiol. 2011; 52(3):578-87. DOI: 10.1093/pcp/pcr016. View

Liu J, Cai J, Wang R, Yang S . Transcriptional Regulation and Transport of Terpenoid Indole Alkaloid in Catharanthus roseus: Exploration of New Research Directions. Int J Mol Sci. 2016; 18(1). PMC: 5297688. DOI: 10.3390/ijms18010053. View

Cloonan N, Forrest A, Kolle G, Gardiner B, Faulkner G, Brown M . Stem cell transcriptome profiling via massive-scale mRNA sequencing. Nat Methods. 2008; 5(7):613-9. DOI: 10.1038/nmeth.1223. View

Papon N, Vansiri A, Gantet P, Chenieux J, Rideau M, Creche J . Histidine-containing phosphotransfer domain extinction by RNA interference turns off a cytokinin signalling circuitry in Catharanthus roseus suspension cells. FEBS Lett. 2004; 558(1-3):85-8. DOI: 10.1016/S0014-5793(03)01522-9. View

Yang M, You W, Wu S, Fan Z, Xu B, Zhu M . Global transcriptome analysis of Huperzia serrata and identification of critical genes involved in the biosynthesis of huperzine A. BMC Genomics. 2017; 18(1):245. PMC: 5361696. DOI: 10.1186/s12864-017-3615-8. View

Lin X, Zhang J, Li Y, Luo H, Wu Q, Sun C . Functional genomics of a living fossil tree, Ginkgo, based on next-generation sequencing technology. Physiol Plant. 2011; 143(3):207-18. DOI: 10.1111/j.1399-3054.2011.01500.x. View

Udomsom N, Rai A, Suzuki H, Okuyama J, Imai R, Mori T . Function of AP2/ERF Transcription Factors Involved in the Regulation of Specialized Metabolism in Revealed by Transcriptomics and Metabolomics. Front Plant Sci. 2016; 7:1861. PMC: 5145908. DOI: 10.3389/fpls.2016.01861. View

Chen T, Wu H, Wu J, Fan X, Li X, Lin Y . Absence of OsβCA1 causes a CO deficit and affects leaf photosynthesis and the stomatal response to CO in rice. Plant J. 2017; 90(2):344-357. DOI: 10.1111/tpj.13497. View

10.

Zhang H, Hedhili S, Montiel G, Zhang Y, Chatel G, Pre M . The basic helix-loop-helix transcription factor CrMYC2 controls the jasmonate-responsive expression of the ORCA genes that regulate alkaloid biosynthesis in Catharanthus roseus. Plant J. 2011; 67(1):61-71. DOI: 10.1111/j.1365-313X.2011.04575.x. View

11.

Livak K, Schmittgen T . Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method. Methods. 2002; 25(4):402-8. DOI: 10.1006/meth.2001.1262. View

12.

Mortazavi A, Williams B, McCue K, Schaeffer L, Wold B . Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008; 5(7):621-8. DOI: 10.1038/nmeth.1226. View

13.

Wilhelm B, Marguerat S, Goodhead I, Bahler J . Defining transcribed regions using RNA-seq. Nat Protoc. 2010; 5(2):255-66. DOI: 10.1038/nprot.2009.229. View

14.

Li C, Leopold A, Sander G, Shanks J, Zhao L, Gibson S . The ORCA2 transcription factor plays a key role in regulation of the terpenoid indole alkaloid pathway. BMC Plant Biol. 2013; 13:155. PMC: 3851283. DOI: 10.1186/1471-2229-13-155. View

15.

Peebles C, Hughes E, Shanks J, San K . Transcriptional response of the terpenoid indole alkaloid pathway to the overexpression of ORCA3 along with jasmonic acid elicitation of Catharanthus roseus hairy roots over time. Metab Eng. 2008; 11(2):76-86. DOI: 10.1016/j.ymben.2008.09.002. View

16.

Verma M, Ghangal R, Sharma R, Sinha A, Jain M . Transcriptome analysis of Catharanthus roseus for gene discovery and expression profiling. PLoS One. 2014; 9(7):e103583. PMC: 4114786. DOI: 10.1371/journal.pone.0103583. View

17.

Wasternack C . Action of jasmonates in plant stress responses and development--applied aspects. Biotechnol Adv. 2013; 32(1):31-9. DOI: 10.1016/j.biotechadv.2013.09.009. View

18.

He B, Gu Y, Xu M, Wang J, Cao F, Xu L . Transcriptome analysis of Ginkgo biloba kernels. Front Plant Sci. 2015; 6:819. PMC: 4593864. DOI: 10.3389/fpls.2015.00819. View

19.

Dinesh-Kumar S, Anandalakshmi R, Marathe R, Schiff M, Liu Y . Virus-induced gene silencing. Methods Mol Biol. 2003; 236:287-94. DOI: 10.1385/1-59259-413-1:287. View

20.

Menke F, Champion A, Kijne J, Memelink J . A novel jasmonate- and elicitor-responsive element in the periwinkle secondary metabolite biosynthetic gene Str interacts with a jasmonate- and elicitor-inducible AP2-domain transcription factor, ORCA2. EMBO J. 1999; 18(16):4455-63. PMC: 1171520. DOI: 10.1093/emboj/18.16.4455. View