Differences in Clinical Aspects of Human Cystic Echinococcosis Caused by and the G6 Genotype in Neuquén, Argentina

Overview

Journal Parasitology

Specialty Parasitology

Date 2023 May 19

PMID 37203451

Authors

Maria Florencia Debiaggi

Cristian A Alvarez Rojas

Lorena Evelina Lazzarini

Daniel Calfunao

Paola Titanti

Liliana Calanni

Marisa Iacono

Silvia Viviana Soriano

Peter Deplazes

Nora Beatriz Pierangeli

Affiliations

Soon will be listed here.

Abstract

Most human cystic echinococcosis (CE) cases worldwide are attributed to (), followed by the G6 and G7 genotypes. While has a cosmopolitan distribution, the G6 genotype is restricted to areas where camels and goats are present. Goats are the primary livestock in the Neuquén province in Argentina where the G6 genotype has been reported to be responsible for a significant percentage of CE human cysts genotyped. In the present study, we genotyped 124 cysts infecting 90 CE-confirmed patients. was identified in 51 patients (56.7%) with 81 cysts and the G6 genotype in 39 patients (43.3%) harbouring 43 cysts. Most CE cases ≤18 years were male suggesting pastoral work could be a risk factor for the infection. was significantly found more frequently in the liver (32/51 patients) and the G6 genotype in the lungs and extrahepatic localizations (27/39). The patients infected with ., presented up to 6 cysts while patients infected with G6 presented a maximum of 2. The diameter of lung cysts attributed to was significantly larger compared to lung cysts from G6. Following the WHO ultrasound classification of liver cysts, we observed inactive cysts in 55.6% of G6 cysts and only 15.3% of cysts. In conclusion, we provide evidence of differences in clinical aspects of CE caused by and the G6 genotype of . .. complex infecting humans.

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References

Kamenetzky L, Gutierrez A, Canova S, Haag K, Guarnera E, Parra A . Several strains of Echinococcus granulosus infect livestock and humans in Argentina. Infect Genet Evol. 2003; 2(2):129-36. DOI: 10.1016/s1567-1348(02)00131-4. View

Maillard S, Gottstein B, Haag K, Ma S, Colovic I, Benchikh-Elfegoun M . The EmsB tandemly repeated multilocus microsatellite: a new tool to investigate genetic diversity of Echinococcus granulosus sensu lato. J Clin Microbiol. 2009; 47(11):3608-16. PMC: 2772617. DOI: 10.1128/JCM.00938-09. View

Pierangeli N, Soriano S, Roccia I, Gimenez J, Lazzarini L, Grenovero M . Heterogeneous distribution of human cystic echinococcosis after a long-term control program in Neuquén, Patagonia Argentina. Parasitol Int. 2007; 56(2):149-55. DOI: 10.1016/j.parint.2007.01.007. View

Laurimae T, Kinkar L, Romig T, Omer R, Casulli A, Umhang G . The benefits of analysing complete mitochondrial genomes: Deep insights into the phylogeny and population structure of Echinococcus granulosus sensu lato genotypes G6 and G7. Infect Genet Evol. 2018; 64:85-94. DOI: 10.1016/j.meegid.2018.06.016. View

Bardonnet K, Piarroux R, Dia L, Schneegans F, Beurdeley A, Godot V . Combined eco-epidemiological and molecular biology approaches to assess Echinococcus granulosus transmission to humans in Mauritania: occurrence of the 'camel' strain and human cystic echinococcosis. Trans R Soc Trop Med Hyg. 2002; 96(4):383-6. DOI: 10.1016/s0035-9203(02)90369-x. View

Brunetti E, Kern P, Vuitton D . Expert consensus for the diagnosis and treatment of cystic and alveolar echinococcosis in humans. Acta Trop. 2009; 114(1):1-16. DOI: 10.1016/j.actatropica.2009.11.001. View

Lahmar S, Rebai W, Boufana B, Craig P, Ksantini R, Daghfous A . Cystic echinococcosis in Tunisia: analysis of hydatid cysts that have been surgically removed from patients. Ann Trop Med Parasitol. 2009; 103(7):593-604. DOI: 10.1179/000349809X12502035776153. View

Orsten S, Ciftci T, Azizova A, Yuce G, Uysal A, Imamoglu C . Investigation of the relationship between CE cyst characteristics and genetic diversity of in humans from Turkey. Parasitology. 2020; 147(14):1712-1717. PMC: 10317744. DOI: 10.1017/S0031182020001535. View

Omer R, Dinkel A, Romig T, Mackenstedt U, Elnahas A, Aradaib I . A molecular survey of cystic echinococcosis in Sudan. Vet Parasitol. 2010; 169(3-4):340-6. DOI: 10.1016/j.vetpar.2010.01.004. View

10.

Bart J, Abdukader M, Zhang Y, Lin R, Wang Y, Nakao M . Genotyping of human cystic echinococcosis in Xinjiang, PR China. Parasitology. 2006; 133(Pt 5):571-9. DOI: 10.1017/S0031182006000734. View

11.

Bart J, Bardonnet K, Elfegoun M, Dumon H, Dia L, Vuitton D . Echinococcus granulosus strain typing in North Africa: comparison of eight nuclear and mitochondrial DNA fragments. Parasitology. 2004; 128(Pt 2):229-34. DOI: 10.1017/s0031182003004359. View

12.

Debiaggi M, Soriano S, Pierangeli N, Lazzarini L, Pianciola L, Mazzeo M . Genetic characterization of human hydatid cysts shows coinfection by Echinococcus canadensis G7 and Echinococcus granulosus sensu stricto G1 in Argentina. Parasitol Res. 2017; 116(9):2599-2604. DOI: 10.1007/s00436-017-5555-2. View

13.

Shahnazi M, Hejazi H, Salehi M, Andalib A . Molecular characterization of human and animal Echinococcus granulosus isolates in Isfahan, Iran. Acta Trop. 2010; 117(1):47-50. DOI: 10.1016/j.actatropica.2010.09.002. View

14.

Casulli A, Zeyhle E, Brunetti E, Pozio E, Meroni V, Genco F . Molecular evidence of the camel strain (G6 genotype) of Echinococcus granulosus in humans from Turkana, Kenya. Trans R Soc Trop Med Hyg. 2009; 104(1):29-32. DOI: 10.1016/j.trstmh.2009.08.001. View

15.

Moro P, Nakao M, Ito A, Schantz P, Cavero C, Cabrera L . Molecular identification of Echinococcus isolates from Peru. Parasitol Int. 2009; 58(2):184-6. DOI: 10.1016/j.parint.2009.01.005. View

16.

Manterola C, Benavente F, Melo A, Vial M, Roa J . Description of Echinococcus granulosus genotypes in human hydatidosis in a region of southern Chile. Parasitol Int. 2008; 57(3):342-6. DOI: 10.1016/j.parint.2008.02.005. View

17.

Guarnera E, Parra A, Kamenetzky L, Garcia G, Gutierrez A . Cystic echinococcosis in Argentina: evolution of metacestode and clinical expression in various Echinococcus granulosus strains. Acta Trop. 2004; 92(2):153-9. DOI: 10.1016/j.actatropica.2004.06.008. View

18.

Alvarez Rojas C, Gauci C, Lightowlers M . Antigenic differences between the EG95-related proteins from Echinococcus granulosus G1 and G6 genotypes: implications for vaccination. Parasite Immunol. 2012; 35(2):99-102. DOI: 10.1111/pim.12009. View

19.

Cucher M, Macchiaroli N, Baldi G, Camicia F, Prada L, Maldonado L . Cystic echinococcosis in South America: systematic review of species and genotypes of Echinococcus granulosus sensu lato in humans and natural domestic hosts. Trop Med Int Health. 2015; 21(2):166-75. DOI: 10.1111/tmi.12647. View

20.

Aaty H, Abdel-Hameed D, Alam-Eldin Y, El-Shennawy S, Aminou H, Makled S . Molecular genotyping of Echinococcus granulosus in animal and human isolates from Egypt. Acta Trop. 2011; 121(2):125-8. DOI: 10.1016/j.actatropica.2011.10.014. View