Identification and Genotyping of from Human Clinical Samples in Guilan Province, North of Iran

Overview

Journal Parasite Epidemiol Control

Specialty Parasitology

Date 2024 May 3

PMID 38699631

Authors

Masoumeh Gholami Koohestan

Reza Saberi

Ahmad Daryani

Shahabbedin Sarvi

Meysam Sharifdini

Davood Anvari

Seyyed Ali Shariatzadeh

Seyed Abdollah Hosseini

Shirzad Gholami

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis (CE) is a significant health problem in both human and veterinary medicine. It is caused by the tapeworm The objective of this study was to investigate molecular diversity of from the paraffin-embedded human (FFPE) tissue samples using sequencing of mitochondrial genes. Thirty-five FFPE tissue samples were collected from different regions of Guilan province, north of Iran. Demographic data were recorded using a questionnaire. Five sections (1 mm) of the tissue were prepared and deparaffined using xylene and ethanol methods. Molecular analysis was performed using the and genes using PCR and DNA sequencing. Totally, 25 cases (71.43%) were women and 10 cases (28.57%) were men. The most affected age group was 21-30 yr old. The most of cysts were isolated from the liver ( = 19; 54.29%) and others in the lung ( = 16; 45.71%). The and genes were successfully amplified in 16 (45.71%) and 12 (34.28%) DNA samples from FFPE tissue. Sequencing analysis revealed that all samples were sensu stricto (G1 and G3) In this study, sensu stricto complex G1 and G3 were identified in human hydatid cysts and showed the presence of sheep/dog cycle in human infection. This finding confirmed and completed previous studies on the geospatial distribution of sensu stricto complex G1 and G3 in the southern and coastal areas of the Caspian Sea region.

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