Antibacterial Activity and Mechanism of Peptide PV-Q5 Against and , Derived from Salt-Fermented

Overview

Journal Foods

Specialty Biotechnology

Date 2023 May 13

PMID 37174342

Authors

Jingyi Dai

Ritian Jin

Jialong Gao

Jude Juventus Aweya

Rong Lin

Guiling Li

Shen Yang

Affiliations

Soon will be listed here.

Abstract

The increasing threat posed by antibiotic-resistant pathogens has prompted a shift to the use of naturally-derived antimicrobial peptides (AMPs) in place of chemical preservatives in controlling foodborne pathogens. In this study, ten peptides were identified from salt-fermented shrimps () using ultra-performance liquid chromatography-mass spectrometry. One of the peptides, designated PV-Q5 (QVRNFPRGSAASPSALASPR), with most features of an AMP, was further explored and found to possess strong antibacterial activity against and , with a minimum inhibitory concentration of 31.25 μg/mL. Moreover, PV-Q5 increased bacterial cell membrane permeability and ruptured bacteria cell membranes, as revealed by transmission electron microscopy. Circular dichroism analysis showed that the conformation of PV-Q5 was a random coil in phosphate-buffered saline and α-helical in sodium dodecyl sulfate, which is conducive for interaction with bacteria cell membranes. These findings indicated that PV-Q5 could find potential use in food preservation to control foodborne pathogenic bacteria.

Citing Articles

Effect of sublethal dose of chloramphenicol on biofilm formation and virulence in .

Zhang M, Cai L, Luo X, Li X, Zhang T, Wu F Front Microbiol. 2023; 14:1275441.

PMID: 37822746 PMC: 10562556. DOI: 10.3389/fmicb.2023.1275441.

References

Liu C, Baffoe D, Zhan Y, Zhang M, Li Y, Zhang G . Halophile, an essential platform for bioproduction. J Microbiol Methods. 2019; 166:105704. DOI: 10.1016/j.mimet.2019.105704. View

Gharsallaoui A, Oulahal N, Joly C, Degraeve P . Nisin as a Food Preservative: Part 1: Physicochemical Properties, Antimicrobial Activity, and Main Uses. Crit Rev Food Sci Nutr. 2015; 56(8):1262-74. DOI: 10.1080/10408398.2013.763765. View

Castellano P, Mora L, Escudero E, Vignolo G, Aznar R, Toldra F . Antilisterial peptides from Spanish dry-cured hams: Purification and identification. Food Microbiol. 2016; 59:133-41. DOI: 10.1016/j.fm.2016.05.018. View

Omidbakhsh Amiri E, Farmani J, Amiri Z, Dehestani A, Mohseni M . Antimicrobial activity, environmental sensitivity, mechanism of action, and food application of αs165-181 peptide. Int J Food Microbiol. 2021; 358:109403. DOI: 10.1016/j.ijfoodmicro.2021.109403. View

Lan Y, Ye Y, Kozlowska J, Lam J, Drake A, Mason A . Structural contributions to the intracellular targeting strategies of antimicrobial peptides. Biochim Biophys Acta. 2010; 1798(10):1934-43. PMC: 3309561. DOI: 10.1016/j.bbamem.2010.07.003. View

Courvalin P . Why is antibiotic resistance a deadly emerging disease?. Clin Microbiol Infect. 2016; 22(5):405-7. DOI: 10.1016/j.cmi.2016.01.012. View

Bingham J, Mitsunaga E, Bergeron Z . Drugs from slugs--past, present and future perspectives of omega-conotoxin research. Chem Biol Interact. 2009; 183(1):1-18. DOI: 10.1016/j.cbi.2009.09.021. View

Abdelhamid A, El-Dougdoug N . Controlling foodborne pathogens with natural antimicrobials by biological control and antivirulence strategies. Heliyon. 2020; 6(9):e05020. PMC: 7511826. DOI: 10.1016/j.heliyon.2020.e05020. View

Alegbeleye O, Singleton I, SantAna A . Sources and contamination routes of microbial pathogens to fresh produce during field cultivation: A review. Food Microbiol. 2018; 73:177-208. PMC: 7127387. DOI: 10.1016/j.fm.2018.01.003. View

10.

Agbale C, Sarfo J, Galyuon I, Juliano S, Silva G, Buccini D . Antimicrobial and Antibiofilm Activities of Helical Antimicrobial Peptide Sequences Incorporating Metal-Binding Motifs. Biochemistry. 2019; 58(36):3802-3812. DOI: 10.1021/acs.biochem.9b00440. View

11.

Wang J, Dou X, Song J, Lyu Y, Zhu X, Xu L . Antimicrobial peptides: Promising alternatives in the post feeding antibiotic era. Med Res Rev. 2018; 39(3):831-859. DOI: 10.1002/med.21542. View

12.

Shelar A, Bansal M . Helix perturbations in membrane proteins assist in inter-helical interactions and optimal helix positioning in the bilayer. Biochim Biophys Acta. 2016; 1858(11):2804-2817. DOI: 10.1016/j.bbamem.2016.08.003. View

13.

Zasloff M . Antimicrobial Peptides of Multicellular Organisms: My Perspective. Adv Exp Med Biol. 2019; 1117:3-6. DOI: 10.1007/978-981-13-3588-4_1. View

14.

Tan T, Wu D, Li W, Zheng X, Li W, Shan A . High Specific Selectivity and Membrane-Active Mechanism of Synthetic Cationic Hybrid Antimicrobial Peptides Based on the Peptide FV7. Int J Mol Sci. 2017; 18(2). PMC: 5343874. DOI: 10.3390/ijms18020339. View

15.

Wang G . Database-Guided Discovery of Potent Peptides to Combat HIV-1 or Superbugs. Pharmaceuticals (Basel). 2013; 6(6):728-58. PMC: 3816732. DOI: 10.3390/ph6060728. View

16.

Raja Z, Andre S, Piesse C, Sereno D, Nicolas P, Foulon T . Structure, antimicrobial activities and mode of interaction with membranes of novel [corrected] phylloseptins from the painted-belly leaf frog, Phyllomedusa sauvagii. PLoS One. 2013; 8(8):e70782. PMC: 3742671. DOI: 10.1371/journal.pone.0070782. View

17.

Nielsen S, Jakobsen L, Geiker N, Bertram H . Chemically acidified, live and heat-inactivated fermented dairy yoghurt show distinct bioactive peptides, free amino acids and small compounds profiles. Food Chem. 2021; 376:131919. DOI: 10.1016/j.foodchem.2021.131919. View

18.

Martinez M, Polizzotto A, Flores N, Semorile L, Maffia P . Antibacterial, anti-biofilm and in vivo activities of the antimicrobial peptides P5 and P6.2. Microb Pathog. 2019; 139:103886. DOI: 10.1016/j.micpath.2019.103886. View

19.

Jiang Y, Chen Y, Song Z, Tan Z, Cheng J . Recent advances in design of antimicrobial peptides and polypeptides toward clinical translation. Adv Drug Deliv Rev. 2021; 170:261-280. DOI: 10.1016/j.addr.2020.12.016. View

20.

Yang S, Yuan Z, Aweya J, Huang S, Deng S, Shi L . Low-intensity ultrasound enhances the antimicrobial activity of neutral peptide TGH2 against Escherichia coli. Ultrason Sonochem. 2021; 77:105676. PMC: 8326391. DOI: 10.1016/j.ultsonch.2021.105676. View