Protein Structural Analysis by Cryogenic Electron Microscopy

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Apr 24

PMID 37093490

Authors

Michael Hall

Erin Schexnaydre

Camilla Holmlund

Marta Carroni

Affiliations

Soon will be listed here.

Abstract

Cryogenic electron microscopy (cryo-EM) is constantly developing and growing as a major technique for structure determination of protein complexes. Here, we detail the first steps of any cryo-EM project: specimen preparation and data collection. Step by step, a list of material needed is provided and the sequence of actions to carry out is given. We hope that these protocols will be useful to all people getting started with cryo-EM.

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