Recent Advances in Vascularized Tumor-on-a-chip

Overview

Journal Front Oncol

Specialty Oncology

Date 2023 Apr 17

PMID 37064144

Authors

Christina Bao Xian Huang

Ting-Yuan Tu

Affiliations

Soon will be listed here.

Abstract

The vasculature plays a critical role in cancer progression and metastasis, representing a pivotal aspect in the creation of cancer models. In recent years, the emergence of organ-on-a-chip technology has proven to be a robust tool, capable of replicating conditions with exceptional spatiotemporal resolution, making it a significant asset in cancer research. This review delves into the latest developments in 3D microfluidic vascularized tumor models and their applications , focusing on heterotypic cellular interactions, the mechanisms of metastasis, and therapeutic screening. Additionally, the review examines the benefits and drawbacks of these models, as well as the future prospects for their advancement.

Citing Articles

Microfluidic model of the alternative vasculature in neuroblastoma.

Villasante A, Lopez-Martinez M, Quinonero G, Garcia-Lizarribar A, Peng X, Samitier J In Vitro Model. 2025; 3(1):49-63.

PMID: 39872066 PMC: 11756480. DOI: 10.1007/s44164-023-00064-x.

In Silico Approach to Model Heat Distribution of Magnetic Hyperthermia in the Tumoral and Healthy Vascular Network Using Tumor-on-a-Chip to Evaluate Effective Therapy.

Munoz J, Pileggi G, Penteado Nucci M, Alves A, Pedrini F, Valle N Pharmaceutics. 2024; 16(9).

PMID: 39339193 PMC: 11434665. DOI: 10.3390/pharmaceutics16091156.

Microphysiological systems as models for immunologically 'cold' tumors.

Gaebler D, Hachey S, Hughes C Front Cell Dev Biol. 2024; 12:1389012.

PMID: 38711620 PMC: 11070549. DOI: 10.3389/fcell.2024.1389012.

Vascularized tumor models for the evaluation of drug delivery systems: a paradigm shift.

Lopez-Vince E, Wilhelm C, Simon-Yarza T Drug Deliv Transl Res. 2024; 14(8):2216-2241.

PMID: 38619704 PMC: 11208221. DOI: 10.1007/s13346-024-01580-3.

References

Braun A, Anders H, Gudermann T, Mammadova-Bach E . Platelet-Cancer Interplay: Molecular Mechanisms and New Therapeutic Avenues. Front Oncol. 2021; 11:665534. PMC: 8311658. DOI: 10.3389/fonc.2021.665534. View

Kim D, Hwang K, Seo E, Seo S, Lee B, Choi N . Vascularized Lung Cancer Model for Evaluating the Promoted Transport of Anticancer Drugs and Immune Cells in an Engineered Tumor Microenvironment. Adv Healthc Mater. 2022; 11(12):e2102581. PMC: 11468795. DOI: 10.1002/adhm.202102581. View

Chen M, Hajal C, Benjamin D, Yu C, Azizgolshani H, Hynes R . Inflamed neutrophils sequestered at entrapped tumor cells via chemotactic confinement promote tumor cell extravasation. Proc Natl Acad Sci U S A. 2018; 115(27):7022-7027. PMC: 6142213. DOI: 10.1073/pnas.1715932115. View

Karaman S, Detmar M . Mechanisms of lymphatic metastasis. J Clin Invest. 2014; 124(3):922-8. PMC: 3938272. DOI: 10.1172/JCI71606. View

Park J, Kim S, Hong J, Jeon J . Enabling perfusion through multicellular tumor spheroids promoting lumenization in a vascularized cancer model. Lab Chip. 2022; 22(22):4335-4348. DOI: 10.1039/d2lc00597b. View

Mitchell M, King M . Computational and experimental models of cancer cell response to fluid shear stress. Front Oncol. 2013; 3:44. PMC: 3587800. DOI: 10.3389/fonc.2013.00044. View

Frose J, Chen M, Hebron K, Reinhardt F, Hajal C, Zijlstra A . Epithelial-Mesenchymal Transition Induces Podocalyxin to Promote Extravasation via Ezrin Signaling. Cell Rep. 2018; 24(4):962-972. PMC: 6181240. DOI: 10.1016/j.celrep.2018.06.092. View

Sontheimer-Phelps A, Hassell B, Ingber D . Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019; 19(2):65-81. DOI: 10.1038/s41568-018-0104-6. View

Wan Z, Zhong A, Zhang S, Pavlou G, Coughlin M, Shelton S . A Robust Method for Perfusable Microvascular Network Formation In Vitro. Small Methods. 2022; 6(6):e2200143. PMC: 9844969. DOI: 10.1002/smtd.202200143. View

10.

Nashimoto Y, Hayashi T, Kunita I, Nakamasu A, Torisawa Y, Nakayama M . Integrating perfusable vascular networks with a three-dimensional tissue in a microfluidic device. Integr Biol (Camb). 2017; 9(6):506-518. DOI: 10.1039/c7ib00024c. View

11.

Lambert A, Pattabiraman D, Weinberg R . Emerging Biological Principles of Metastasis. Cell. 2017; 168(4):670-691. PMC: 5308465. DOI: 10.1016/j.cell.2016.11.037. View

12.

Gilardi M, Bersini S, Valtorta S, Proietto M, Crippa M, Boussommier-Calleja A . The driving role of the Cdk5/Tln1/FAK axis in cancer cell extravasation dissected by human vascularized microfluidic models. Biomaterials. 2021; 276:120975. DOI: 10.1016/j.biomaterials.2021.120975. View

13.

Hu Z, Cao Y, Galan E, Hao L, Zhao H, Tang J . Vascularized Tumor Spheroid-on-a-Chip Model Verifies Synergistic Vasoprotective and Chemotherapeutic Effects. ACS Biomater Sci Eng. 2022; 8(3):1215-1225. DOI: 10.1021/acsbiomaterials.1c01099. View

14.

Boussommier-Calleja A, Atiyas Y, Haase K, Headley M, Lewis C, Kamm R . The effects of monocytes on tumor cell extravasation in a 3D vascularized microfluidic model. Biomaterials. 2018; 198:180-193. PMC: 6123301. DOI: 10.1016/j.biomaterials.2018.03.005. View

15.

Fernandez-Cortes M, Delgado-Bellido D, Oliver F . Vasculogenic Mimicry: Become an Endothelial Cell "But Not So Much". Front Oncol. 2019; 9:803. PMC: 6714586. DOI: 10.3389/fonc.2019.00803. View

16.

Brown A, He H, Trumper E, Valdez J, Hammond P, Griffith L . Engineering PEG-based hydrogels to foster efficient endothelial network formation in free-swelling and confined microenvironments. Biomaterials. 2020; 243:119921. PMC: 7203641. DOI: 10.1016/j.biomaterials.2020.119921. View

17.

Hassell B, Goyal G, Lee E, Sontheimer-Phelps A, Levy O, Chen C . Human Organ Chip Models Recapitulate Orthotopic Lung Cancer Growth, Therapeutic Responses, and Tumor Dormancy In Vitro. Cell Rep. 2017; 21(2):508-516. DOI: 10.1016/j.celrep.2017.09.043. View

18.

Kim J, Chung M, Kim S, Jo D, Kim J, Jeon N . Engineering of a Biomimetic Pericyte-Covered 3D Microvascular Network. PLoS One. 2015; 10(7):e0133880. PMC: 4512698. DOI: 10.1371/journal.pone.0133880. View

19.

Chen M, Whisler J, Frose J, Yu C, Shin Y, Kamm R . On-chip human microvasculature assay for visualization and quantification of tumor cell extravasation dynamics. Nat Protoc. 2017; 12(5):865-880. PMC: 5509465. DOI: 10.1038/nprot.2017.018. View

20.

Liu Y, Sakolish C, Chen Z, Phan D, Bender R, Hughes C . Human in vitro vascularized micro-organ and micro-tumor models are reproducible organ-on-a-chip platforms for studies of anticancer drugs. Toxicology. 2020; 445:152601. PMC: 7606810. DOI: 10.1016/j.tox.2020.152601. View