A Robust Method for Perfusable Microvascular Network Formation In Vitro

Overview

Journal Small Methods

Specialty Biotechnology

Date 2022 Apr 4

PMID 35373502

Authors

Zhengpeng Wan

Amy X Zhong

Shun Zhang

Georgios Pavlou

Mark F Coughlin

Sarah E Shelton

Huu Tuan Nguyen

Jochen H Lorch

David A Barbie

Roger D Kamm

Affiliations

Soon will be listed here.

Abstract

Micropost-based microfluidic devices are widely used for microvascular network (MVN) formation in diverse research fields. However, consistently generating perfusable MVNs of physiological morphology and dimension has proven to be challenging. Here, how initial seeding parameters determine key characteristics of MVN formation is investigated and a robust two-step seeding strategy to generate perfusable physiological MVNs in microfluidic devices is established.

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