Chicken-origin Cluster 3.2 Tembusu Virus Exhibits Higher Infectivity Than Duck-origin Cluster 2 Tembusu Virus in Chicks

Overview

Journal Front Vet Sci

Specialty Veterinary Medicine

Date 2023 Apr 10

PMID 37035806

Authors

Yunzhen Huang

Ziqiang Hu

Jiawen Dong

Linlin Li

Junqin Zhang

Ruihuan Kuang

Shimin Gao

Minhua Sun

Ming Liao

Affiliations

Soon will be listed here.

Abstract

In 2020, a chicken-origin Cluster 3 Tembusu virus (TMUV) caused outbreaks of a disease characterized by egg-drop syndrome in laying hens in China. In the present study, a TMUV strain, TMUV-GX, was isolated from tissue samples of laying hens with egg drop syndrome in south China. Phylogenetic analysis grouped TMUV-GX into TMUV Cluster 3.2, which was distinct from the prevalent TMUV Cluster 2 in duck flocks. To study the infectivity and pathogenicity of TMUV-GX in chickens and ducks, 7 day-old specific pathogen-free (SPF) chicks and SPF ducklings were infected with the same dose of the TMUV-GX. As a comparison, the duck-origin Cluster 2 strain, TMUV-JM, infection groups were set up in chicks and ducklings. Compared with the low infectivity and pathogenicity of TMUV-JM in chicks, the chicken-origin TMUV-GX displayed high replication competence in multiple tissues and caused tissues histopathological damage. In addition, the replication competence of TMUV-GX in ducklings was comparable to that of TMUV-JM. Our study revealed chicken-origin Cluster 3.2 TMUV exhibits high infectivity in chicks and ducklings, and suggested that chicken-origin Cluster 3.2 TMUV possesses a biological basis for widespread infection of chickens and ducks.

Citing Articles

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Pathogenicity and Interspecies Transmission of Cluster 3 Tembusu Virus Strain TMUV HQ-22 Isolated from Geese.

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References

Su J, Li S, Hu X, Yu X, Wang Y, Liu P . Duck egg-drop syndrome caused by BYD virus, a new Tembusu-related flavivirus. PLoS One. 2011; 6(3):e18106. PMC: 3063797. DOI: 10.1371/journal.pone.0018106. View

Yu Z, Ren H, Sun M, Xie W, Sun S, Liang N . Tembusu virus infection in laying chickens: Evidence for a distinct genetic cluster with significant antigenic variation. Transbound Emerg Dis. 2021; 69(4):e1130-e1141. DOI: 10.1111/tbed.14402. View

Li X, Shi Y, Liu Q, Wang Y, Li G, Teng Q . Airborne Transmission of a Novel Tembusu Virus in Ducks. J Clin Microbiol. 2015; 53(8):2734-6. PMC: 4508455. DOI: 10.1128/JCM.00770-15. View

Yu G, Lin Y, Tang Y, Diao Y . Evolution of Tembusu Virus in Ducks, Chickens, Geese, Sparrows, and Mosquitoes in Northern China. Viruses. 2018; 10(9). PMC: 6164154. DOI: 10.3390/v10090485. View

Cao Z, Zhang C, Liu Y, Ye W, Han J, Ma G . Tembusu virus in ducks, china. Emerg Infect Dis. 2011; 17(10):1873-5. PMC: 3310660. DOI: 10.3201/eid1710.101890. View

Kono Y, Tsukamoto K, Abd Hamid M, Darus A, Lian T, Sam L . Encephalitis and retarded growth of chicks caused by Sitiawan virus, a new isolate belonging to the genus Flavivirus. Am J Trop Med Hyg. 2001; 63(1-2):94-101. DOI: 10.4269/ajtmh.2000.63.94. View

Xu T, Liu X, Liu Q, Han K, Liu Y, Jang J . Effect of experimental infections of various Tembusu virus strains isolated from geese, ducks and chickens on ducklings. Pol J Vet Sci. 2018; 21(2):389-396. DOI: 10.24425/122613. View

Liu M, Chen S, Chen Y, Liu C, Chen S, Yin X . Adapted Tembusu-like virus in chickens and geese in China. J Clin Microbiol. 2012; 50(8):2807-9. PMC: 3421502. DOI: 10.1128/JCM.00655-12. View

Sun M, Dong J, Li L, Lin Q, Sun J, Liu Z . Recombinant Newcastle disease virus (NDV) expressing Duck Tembusu virus (DTMUV) pre-membrane and envelope proteins protects ducks against DTMUV and NDV challenge. Vet Microbiol. 2018; 218:60-69. PMC: 7117350. DOI: 10.1016/j.vetmic.2018.03.027. View

10.

Li L, Zhang Y, Dong J, Zhang J, Zhang C, Sun M . The truncated E protein of DTMUV provide protection in young ducks. Vet Microbiol. 2020; 240:108508. DOI: 10.1016/j.vetmic.2019.108508. View

11.

Yan D, Li X, Wang Z, Liu X, Dong X, Fu R . The emergence of a disease caused by a mosquito origin Cluster 3.2 Tembusu virus in chickens in China. Vet Microbiol. 2022; 272:109500. DOI: 10.1016/j.vetmic.2022.109500. View

12.

Yan D, Wang B, Shi Y, Ni X, Wu X, Li X . A Single Mutation at Position 120 in the Envelope Protein Attenuates Tembusu Virus in Ducks. Viruses. 2022; 14(3). PMC: 8951291. DOI: 10.3390/v14030447. View

13.

Kumar S, Stecher G, Tamura K . MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol. 2016; 33(7):1870-4. PMC: 8210823. DOI: 10.1093/molbev/msw054. View

14.

Ninvilai P, Limcharoen B, Tunterak W, Prakairungnamthip D, Oraveerakul K, Banlunara W . Pathogenesis of Thai duck Tembusu virus in Cherry Valley ducks: The effect of age on susceptibility to infection. Vet Microbiol. 2020; 243:108636. DOI: 10.1016/j.vetmic.2020.108636. View

15.

Thontiravong A, Ninvilai P, Tunterak W, Nonthabenjawan N, Chaiyavong S, Angkabkingkaew K . Tembusu-Related Flavivirus in Ducks, Thailand. Emerg Infect Dis. 2015; 21(12):2164-7. PMC: 4672441. DOI: 10.3201/eid2112.150600. View

16.

Kuno G, Chang G, Tsuchiya K, KARABATSOS N, Cropp C . Phylogeny of the genus Flavivirus. J Virol. 1998; 72(1):73-83. PMC: 109351. DOI: 10.1128/JVI.72.1.73-83.1998. View