A Single Mutation at Position 120 in the Envelope Protein Attenuates Tembusu Virus in Ducks

Overview

Journal Viruses

Publisher MDPI

Specialty Microbiology

Date 2022 Mar 26

PMID 35336854

Authors

Dawei Yan

Binbin Wang

Ying Shi

Xintao Ni

Xiaogang Wu

Xuesong Li

Xingpo Liu

Haiwang Wang

Xin Su

Qiaoyang Teng

Jianmei Yang

Qinfang Liu

Zejun Li

Affiliations

Soon will be listed here.

Abstract

A live attenuated duck Tembusu virus (TMUV) vaccine FX2010-180P (180P) was successfully utilized to prevent TMUV infections in ducks in China. Compared with wild-type TMUV, 180P was highly attenuated and lost transmissibility in ducks. However, the mechanism of the attenuation of 180P remains poorly understood. To explore the key molecular basis of attenuation, chimeric and site mutant viruses in the background of the wild-type TMUV-FX2010 (FX) strain were rescued, and the replication, tissue tropism, and transmissibility were characterized in ducks. The results show that the envelope (E) protein was responsible for attenuation and loss of transmission in ducks. Further studies showed that a D120N amino acid mutation located in domain II of the E protein was responsible for the attenuation and transmissibility loss of 180P in ducks. The D120N substitution resulted in an extra high-mannose type N-linked glycosylation (NLG) in the E protein of 180P compared with the wild-type TMUV, which might restrict the tissue tropism and transmissibility of TMUV in ducks. Our findings elucidate that N120 in the E protein is a key molecular basis of TMUV attenuation in ducks and provide new insight into the role of NLG in TMUV tissue tropism and transmissibility.

Citing Articles

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