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Engineering the Maturation of Stem Cell-derived Cardiomyocytes

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Date 2023 Apr 10
PMID 37034258
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Abstract

The maturation of human stem cell-derived cardiomyocytes (hSC-CMs) has been a major challenge to further expand the scope of their application. Over the past years, several strategies have been proven to facilitate the structural and functional maturation of hSC-CMs, which include but are not limited to engineering the geometry or stiffness of substrates, providing favorable extracellular matrices, applying mechanical stretch, fluidic or electrical stimulation, co-culturing with niche cells, regulating biochemical cues such as hormones and transcription factors, engineering and redirecting metabolic patterns, developing 3D cardiac constructs such as cardiac organoid or engineered heart tissue, or culturing under implantation. In this review, we summarize these maturation strategies, especially the recent advancements, and discussed their advantages as well as the pressing problems that need to be addressed in future studies.

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References
1.
Kadota S, Pabon L, Reinecke H, Murry C . In Vivo Maturation of Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes in Neonatal and Adult Rat Hearts. Stem Cell Reports. 2017; 8(2):278-289. PMC: 5311430. DOI: 10.1016/j.stemcr.2016.10.009. View

2.
Feaster T, Cadar A, Wang L, Williams C, Chun Y, Hempel J . Matrigel Mattress: A Method for the Generation of Single Contracting Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Circ Res. 2015; 117(12):995-1000. PMC: 4670592. DOI: 10.1161/CIRCRESAHA.115.307580. View

3.
Emanuelli G, Zoccarato A, Reumiller C, Papadopoulos A, Chong M, Rebs S . A roadmap for the characterization of energy metabolism in human cardiomyocytes derived from induced pluripotent stem cells. J Mol Cell Cardiol. 2021; 164:136-147. DOI: 10.1016/j.yjmcc.2021.12.001. View

4.
Correia C, Koshkin A, Duarte P, Hu D, Teixeira A, Domian I . Distinct carbon sources affect structural and functional maturation of cardiomyocytes derived from human pluripotent stem cells. Sci Rep. 2017; 7(1):8590. PMC: 5561128. DOI: 10.1038/s41598-017-08713-4. View

5.
Maleckar M, Edwards A, Louch W, Lines G . Studying dyadic structure-function relationships: a review of current modeling approaches and new insights into Ca (mis)handling. Clin Med Insights Cardiol. 2017; 11:1179546817698602. PMC: 5392018. DOI: 10.1177/1179546817698602. View