Evaluation of Biofilm Cultivation Models for Efficacy Testing of Disinfectants Against Typhimurium Biofilms

Overview

Journal Microorganisms

Specialty Microbiology

Date 2023 Mar 29

PMID 36985334

Authors

Anja M Richter

Katharina Konrat

Ane M Osland

Emma Brook

Claire Oastler

Lene K Vestby

Rebecca J Gosling

Live L Nesse

Mardjan Arvand

Affiliations

Soon will be listed here.

Abstract

Within the European Union, is frequently reported in food and feed products. A major route of transmission is upon contact with contaminated surfaces. In nature, bacteria such as are often encountered in biofilms, where they are protected against antibiotics and disinfectants. Therefore, the removal and inactivation of biofilms is essential to ensure hygienic conditions. Currently, recommendations for disinfectant usage are based on results of efficacy testing against planktonic bacteria. There are no biofilm-specific standards for the efficacy testing of disinfectants against . Here, we assessed three models for disinfectant efficacy testing on Typhimurium biofilms. Achievable bacterial counts per biofilm, repeatability, and intra-laboratory reproducibility were analyzed. Biofilms of two strains were grown on different surfaces and treated with glutaraldehyde or peracetic acid. Disinfectant efficacy was compared with results for planktonic . All methods resulted in highly repeatable cell numbers per biofilm, with one assay showing variations of less than 1 log CFU in all experiments for both strains tested. Disinfectant concentrations required to inactivate biofilms were higher compared to planktonic cells. Differences were found between the biofilm methods regarding maximal achievable cell numbers, repeatability, and intra-laboratory reproducibility of results, which may be used to identify the most appropriate method in relation to application context. Developing a standardized protocol for testing disinfectant efficacy on biofilms will help identify conditions that are effective against biofilms.

Citing Articles

Evaluation of Disinfectant Efficacy against Biofilm-Residing Wild-Type from the Porcine Industry.

Osland A, Oastler C, Konrat K, Nesse L, Brook E, Richter A Antibiotics (Basel). 2023; 12(7).

PMID: 37508285 PMC: 10376135. DOI: 10.3390/antibiotics12071189.

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