Stunning of Neutrophils Accounts for the Anti-inflammatory Effects of Clodronate Liposomes

Overview

Journal J Exp Med

Specialties Allergy & Immunology
General Medicine

Date 2023 Mar 28

PMID 36976180

Authors

Stephan Culemann

Katharina Knab

Maximilien Euler

Anja Wegner

Hilal Garibagaoglu

Jochen Ackermann

Kim Fischer

Deborah Kienhofer

Georgiana Crainiciuc

Jonas Hahn

Anika Gruneboom

Falk Nimmerjahn

Stefan Uderhardt

Andres Hidalgo

Georg Schett

Markus H Hoffmann

Gerhard Kronke

Affiliations

Soon will be listed here.

Abstract

Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) to study the function of these cells in vivo. Here, we revisited the effects of Clo-Lip together with genetic models of MoPh deficiency, revealing that Clo-Lip exert their anti-inflammatory effects independent of MoPh. Notably, not only MoPh but also polymorphonuclear neutrophils (PMN) ingested Clo-Lip in vivo, which resulted in their functional arrest. Adoptive transfer of PMN, but not of MoPh, reversed the anti-inflammatory effects of Clo-Lip treatment, indicating that stunning of PMN rather than depletion of MoPh accounts for the anti-inflammatory effects of Clo-Lip in vivo. Our data highlight the need for a critical revision of the current literature on the role of MoPh in inflammation.

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