Insights into the Mechanisms of Triptolide Nephrotoxicity Through Network Pharmacology-based Analysis and RNA-seq

Overview

Journal Front Plant Sci

Date 2023 Mar 24

PMID 36959927

Authors

Yue-Ming Luo

Shu-Dong Yang

Miao-Yu Wen

Bing Wang

Jia-Hui Liu

Si-Ting Li

Yu-Yan Li

Hong Cheng

Li-Li Zhao

Shun-Min Li

Jian-Jun Jiang

Affiliations

Soon will be listed here.

Abstract

Introduction: Triptolide (TPL) is a promising plant-derived compound for clinical therapy of multiple human diseases; however, its application was limited considering its toxicity.

Methods: To explore the underlying molecular mechanism of TPL nephrotoxicity, a network pharmacology based approach was utilized to predict candidate targets related with TPL toxicity, followed by deep RNA-seq analysis to characterize the features of three transcriptional elements include protein coding genes (PCGs), long noncoding RNAs (lncRNAs) and circular RNAs (circRNAs) as well as their associations with nephrotoxicity in rats with TPL treatment.

Results & Discussion: Although the deeper mechanisms of TPL nephrotoxcity remain further exploration, our results suggested that c-Jun is a potential target of TPL and Per1 related circadian rhythm signaling is involved in TPL induced renal toxicity.

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