Targeted Deletion of Dicer from Proximal Tubules Protects Against Renal Ischemia-reperfusion Injury

Overview

Journal J Am Soc Nephrol

Specialty Nephrology

Date 2010 Apr 3

PMID 20360310

Citations 133

Authors

Qingqing Wei

Kirti Bhatt

Hong-Zhi He

Qing-Sheng Mi

Volker H Haase

Zheng Dong

Affiliations

Soon will be listed here.

Abstract

MicroRNAs are endogenous, noncoding, small RNAs that regulate expression and function of genes, but little is known about regulation of microRNA in the kidneys under normal or pathologic states. Here, we generated a mouse model in which the proximal tubular cells lack Dicer, a key enzyme for microRNA production. These mice had normal renal function and histology under control conditions despite a global downregulation of microRNAs in the renal cortex; however, these animals were remarkably resistant to renal ischemia-reperfusion injury (IRI), showing significantly better renal function, less tissue damage, lower tubular apoptosis, and improved survival compared with their wild-type littermates. Microarray analysis showed altered expression of specific microRNAs during renal IRI. Taken together, these results demonstrate evidence for a pathogenic role of Dicer and associated microRNAs in renal IRI.

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