Applying CRISPR-Cas9 Genome Editing to Study Genes Involved in Peroxisome Biogenesis or Peroxisomal Functions

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2023 Mar 23

PMID 36952190

Authors

Serhii Chornyi

Janet Koster

Hans R Waterham

Affiliations

Soon will be listed here.

Abstract

The development and application of the CRISPR-Cas9 technology for genome editing of mammalian cells have opened up a wealth of possibilities for genetically modifying and manipulating human cells, and use in functional studies or therapeutic approaches.Here we describe the approach that we have been using successfully to generate multiple human cell lines with targeted (partial) gene deletions, i.e., knockout cells, or human cells with modified genomic nucleotide sequences, i.e., knock-in cells, in genes encoding known or putative proteins involved in peroxisome biogenesis or peroxisomal functions.

Citing Articles

Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells.

Costa C, Lismont C, Chornyi S, Li H, Hussein M, Waterham H Antioxidants (Basel). 2023; 12(6).

PMID: 37371965 PMC: 10295636. DOI: 10.3390/antiox12061236.

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