CRISPR RNA-guided Activation of Endogenous Human Genes

Overview

Journal Nat Methods

Specialties Biomedical Engineering
Pathology

Date 2013 Jul 30

PMID 23892898

Citations 602

Authors

Morgan L Maeder

Samantha J Linder

Vincent M Cascio

Yanfang Fu

Quan H Ho

J Keith Joung

Affiliations

Soon will be listed here.

Abstract

Short guide RNAs (gRNAs) can direct catalytically inactive CRISPR-associated 9 nuclease (dCas9) to repress endogenous genes in bacteria and human cells. Here we show that single or multiple gRNAs can direct dCas9 fused to a VP64 transcriptional activation domain to increase expression of endogenous human genes. This proof-of-principle work shows that clustered regularly interspaced short palindromic repeat (CRISPR)-Cas systems can target heterologous effector domains to endogenous sites in human cells.

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