Molecular Insights into the Catalytic Mechanism of Plasticizer Degradation by a Monoalkyl Phthalate Hydrolase

Overview

Journal Commun Chem

Publisher Springer Nature

Specialty Chemistry

Date 2023 Mar 1

PMID 36859434

Authors

Yebao Chen

Yongjin Wang

Yang Xu

Jiaojiao Sun

Liu Yang

Chenhao Feng

Jia Wang

Yang Zhou

Zhi-Min Zhang

Yonghua Wang

Affiliations

Soon will be listed here.

Abstract

Phthalate acid esters (PAEs), a group of xenobiotic compounds used extensively as plasticizers, have attracted increasing concern for adverse effects to human health and the environment. Microbial degradation relying on PAE hydrolases is a promising treatment. However, only a limited number of PAE hydrolases were characterized to date. Here we report the structures of MehpH, a monoalkyl phthalate (MBP) hydrolase that catalyzes the reaction of MBP to phthalic acid and the corresponding alcohol, in apo and ligand-bound form. The structures reveal a positively-charged catalytic center, complementary to the negatively-charged carboxyl group on MBP, and a penetrating tunnel that serves as exit of alcohol. The study provides a first glimpse into the enzyme-substrate binding model for PAE hydrolases, leading strong support to the development of better enzymes in the future.

Citing Articles

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