A Fluorescent Biosensor for Streptavidin Detection Based on Double-Hairpin DNA-Templated Copper Nanoparticles

Overview

Journal Biosensors (Basel)

Specialty Biotechnology

Date 2023 Feb 25

PMID 36831934

Authors

Qiangsheng Xiao

Mingjian Chen

Wanpin Nie

Fengjiao Xie

Xiao Yu

Changbei Ma

Affiliations

Soon will be listed here.

Abstract

In this paper, we developed a sensitive, label-free and facile fluorescent strategy for detecting streptavidin (SA) based on double-hairpin DNA-templated copper nanoparticles (CuNPs) and terminal protection of small molecule-linked DNA. Herein, a special DNA hairpin probe was designed and synthesized, which contained two poly T single-stranded loops and a nick point in the middle of the stem. Inspired by the concept of the terminal protection interaction, the specific binding of SA to the biotinylated DNA probe can prevent the exonuclease degradation and keep the integrity of DNA probe, which can be used for synthesizing fluorescent CuNPs as a template. Conversely, the DNA probe would be digested by exonucleases and therefore, would fail to form CuNPs without SA. After systematic optimization, the detection range of SA concentration is from 0.5 to 150 nM with a low detection limit of 0.09 nM. Additionally, the proposed method was also successfully applied in the biological samples. Finally, the proposed method is sensitive, effective and simple, and can be potentially applied for predicting diseases and discovering new drugs.

References

Zhang X, Liu Q, Jin Y, Li B . Determination of the activity of T4 polynucleotide kinase phosphatase by exploiting the sequence-dependent fluorescence of DNA-templated copper nanoclusters. Mikrochim Acta. 2018; 186(1):3. DOI: 10.1007/s00604-018-3102-1. View

Qing T, He X, He D, Ye X, Shangguan J, Liu J . Dumbbell DNA-templated CuNPs as a nano-fluorescent probe for detection of enzymes involved in ligase-mediated DNA repair. Biosens Bioelectron. 2017; 94:456-463. DOI: 10.1016/j.bios.2017.03.035. View

He Y, Jiao B . DNA covalently linked to graphene oxide for biotin-streptavidin interaction assay. Talanta. 2016; 163:140-145. DOI: 10.1016/j.talanta.2016.10.096. View

Lee C, Kim H, Kim S, Park K, Park H . A simple and sensitive detection of small molecule-protein interactions based on terminal protection-mediated exponential strand displacement amplification. Analyst. 2018; 143(9):2023-2028. DOI: 10.1039/c8an00099a. View

Zhuang Y, Chiang P, Wang C, Tan K . Environment-sensitive fluorescent turn-on probes targeting hydrophobic ligand-binding domains for selective protein detection. Angew Chem Int Ed Engl. 2013; 52(31):8124-8. DOI: 10.1002/anie.201302884. View

Wang Q, Jiang B, Xu J, Xie J, Xiang Y, Yuan R . Amplified terminal protection assay of small molecule/protein interactions via a highly characteristic solid-state Ag/AgCl process. Biosens Bioelectron. 2013; 43:19-24. DOI: 10.1016/j.bios.2012.11.035. View

Sun Q, Qian J, Tian H, Duan L, Zhang W . Rational design of biotinylated probes: fluorescent turn-on detection of (strept)avidin and bioimaging in cancer cells. Chem Commun (Camb). 2014; 50(62):8518-21. DOI: 10.1039/c4cc03315a. View

Chen X, Lin C, Chen Y, Luo F, Wang Y, Chen X . Terminal protection of a small molecule-linked loop DNA probe for turn-on label-free fluorescence detection of proteins. Biosens Bioelectron. 2016; 83:97-101. DOI: 10.1016/j.bios.2016.04.038. View

Zhang L, Cai Q, Li J, Ge J, Wang J, Dong Z . A label-free method for detecting biothiols based on poly(thymine)-templated copper nanoparticles. Biosens Bioelectron. 2015; 69:77-82. DOI: 10.1016/j.bios.2015.02.012. View

10.

Zhu W, Dai L, Liu Z, Yang W, Zhao C, Li Y . Hairpin-shaped DNA Templated Copper Nanoparticles for Fluorescence Detection of Adenosine Triphosphate Based on Ligation-mediated Exonuclease Cleavage. Anal Sci. 2017; 33(2):203-207. DOI: 10.2116/analsci.33.203. View

11.

Focsan M, Campu A, Craciun A, Potara M, Leordean C, Maniu D . A simple and efficient design to improve the detection of biotin-streptavidin interaction with plasmonic nanobiosensors. Biosens Bioelectron. 2016; 86:728-735. DOI: 10.1016/j.bios.2016.07.054. View

12.

He Y, Tian F, Zhou J, Jiao B . A fluorescent aptasensor for ochratoxin A detection based on enzymatically generated copper nanoparticles with a polythymine scaffold. Mikrochim Acta. 2019; 186(3):199. DOI: 10.1007/s00604-019-3314-z. View

13.

Seto H, Yamashita C, Kamba S, Kondo T, Hasegawa M, Matsuno M . Biotinylation of silicon and nickel surfaces and detection of streptavidin as biosensor. Langmuir. 2013; 29(30):9457-63. DOI: 10.1021/la401068n. View

14.

Cao J, Wang W, Bo B, Mao X, Wang K, Zhu X . A dual-signal strategy for the solid detection of both small molecules and proteins based on magnetic separation and highly fluorescent copper nanoclusters. Biosens Bioelectron. 2016; 90:534-541. DOI: 10.1016/j.bios.2016.10.021. View

15.

Wang H, Zhang H, Chen Y, Liu Y . A fluorescent biosensor for protein detection based on poly(thymine)-templated copper nanoparticles and terminal protection of small molecule-linked DNA. Biosens Bioelectron. 2015; 74:581-6. DOI: 10.1016/j.bios.2015.07.021. View

16.

Xu F, Shi H, He X, Wang K, He D, Guo Q . Concatemeric dsDNA-templated copper nanoparticles strategy with improved sensitivity and stability based on rolling circle replication and its application in microRNA detection. Anal Chem. 2014; 86(14):6976-82. DOI: 10.1021/ac500955r. View

17.

Qing Z, He X, He D, Wang K, Xu F, Qing T . Poly(thymine)-templated selective formation of fluorescent copper nanoparticles. Angew Chem Int Ed Engl. 2013; 52(37):9719-22. DOI: 10.1002/anie.201304631. View

18.

Kim S, Kim J, Kwon W, Hwang S, Cha B, Kim J . Synthesis of DNA-templated copper nanoparticles with enhanced fluorescence stability for cellular imaging. Mikrochim Acta. 2019; 186(7):479. DOI: 10.1007/s00604-019-3620-5. View

19.

Xu F, Yang T, Chen Y . Quantification of microRNA by DNA-Peptide Probe and Liquid Chromatography-Tandem Mass Spectrometry-Based Quasi-Targeted Proteomics. Anal Chem. 2015; 88(1):754-63. DOI: 10.1021/acs.analchem.5b03056. View

20.

Chen M, Li W, Ma C, Wu K, He H, Wang K . Fluorometric determination of the activity of uracil-DNA glycosylase by using graphene oxide and exonuclease I assisted signal amplification. Mikrochim Acta. 2019; 186(2):110. DOI: 10.1007/s00604-019-3247-6. View