A Fluorescent Aptasensor for Ochratoxin A Detection Based on Enzymatically Generated Copper Nanoparticles with a Polythymine Scaffold

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2019 Feb 24

PMID 30796615

Citations 12

Authors

Yue He

Fengyu Tian

Jing Zhou

Bining Jiao

Affiliations

Soon will be listed here.

Abstract

A fluorescence enhancement method is presented for the determination of ochratoxin A (OTA). The interaction of OTA with its aptamer causes structural changes which, in turn, change fluorescence of enzymatically generated polythymine-coated copper nanoparticles (CuNPs) (with excitation/emission maxima at 340/625 nm). The OTA-binding aptamer was immobilized on magnetic beads. When it binds OTA, it is partially released and exposes a region with a partly complimentary DNA strand (cDNA). After magnetic separation, the cDNA was employed as a primer to trigger the terminal deoxynucleotidyl transferase-mediated polymerization. This process generates polythymine which act as a template for synthesis of the CuNPs. The method is sensitive in having a 2.0 nM detection limit for OTA. It was successfully applied to the determination of OTA in spiked diluted red wine. Graphical abstract Schematic presentation of a fluorometric enhancement method for ochratoxin A assay based on ochratoxin A inducing structure switching of its aptamer and enzymatically generated polythymine for copper nanoparticles formation.

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