Acyl-chain Saturation Regulates the Order of Phosphatidylinositol 4,5-bisphosphate Nanodomains

Overview

Journal Commun Chem

Publisher Springer Nature

Specialty Chemistry

Date 2023 Jan 25

PMID 36697613

Authors

Luis Borges-Araujo

Marco M Domingues

Alexander Fedorov

Nuno C Santos

Manuel N Melo

Fabio Fernandes

Affiliations

Soon will be listed here.

Abstract

Phosphatidylinositol 4,5-bisphosphate (PI(4,5)P) plays a critical role in the regulation of various plasma membrane processes and signaling pathways in eukaryotes. A significant amount of cellular resources are spent on maintaining the dominant 1-stearoyl-2-arachidonyl PI(4,5)P acyl-chain composition, while less abundant and more saturated species become more prevalent in response to specific stimuli, stress or aging. Here, we report the impact of acyl-chain structure on the biophysical properties of cation-induced PI(4,5)P nanodomains. PI(4,5)P species with increasing levels of acyl-chain saturation cluster in progressively more ordered nanodomains, culminating in the formation of gel-like nanodomains for fully saturated species. The formation of these gel-like domains was largely abrogated in the presence of 1-stearoyl-2-arachidonyl PI(4,5)P This is, to the best of our knowledge, the first report of the impact of PI(4,5)P acyl-chain composition on cation-dependent nanodomain ordering, and provides important clues to the motives behind the enrichment of PI(4,5)P with polyunsaturated acyl-chains. We also show how Ca-induced PI(4,5)P nanodomains are able to generate local negative curvature, a phenomenon likely to play a role in membrane remodeling events.

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