Acyl Chain Asymmetry and Polyunsaturation of Brain Phospholipids Facilitate Membrane Vesiculation Without Leakage

Overview

Journal Elife

Specialty Biology

Date 2018 Mar 16

PMID 29543154

Citations 53

Authors

Marco M Manni

Marion L Tiberti

Sophie Pagnotta

Helene Barelli

Romain Gautier

Bruno Antonny

Affiliations

Soon will be listed here.

Abstract

Phospholipid membranes form cellular barriers but need to be flexible enough to divide by fission. Phospholipids generally contain a saturated fatty acid (FA) at position whereas the -FA is saturated, monounsaturated or polyunsaturated. Our understanding of the impact of phospholipid unsaturation on membrane flexibility and fission is fragmentary. Here, we provide a comprehensive view of the effects of the FA profile of phospholipids on membrane vesiculation by dynamin and endophilin. Coupled to simulations, this analysis indicates that: (i) phospholipids with two polyunsaturated FAs make membranes prone to vesiculation but highly permeable; (ii) asymmetric -saturated--polyunsaturated phospholipids provide a tradeoff between efficient membrane vesiculation and low membrane permeability; (iii) When incorporated into phospholipids, docosahexaenoic acid (DHA; omega-3) makes membranes more deformable than arachidonic acid (omega-6). These results suggest an explanation for the abundance of -saturated--DHA phospholipids in synaptic membranes and for the importance of the omega-6/omega-3 ratio on neuronal functions.

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