Dynamic Degradation Patterns of Porous Polycaprolactone/β-tricalcium Phosphate Composites Orchestrate Macrophage Responses and Immunoregulatory Bone Regeneration

Overview

Journal Bioact Mater

Specialty Biomedical Engineering

Date 2023 Jan 23

PMID 36685731

Authors

Hao Wu

Xinghui Wei

Yichao Liu

Hui Dong

Zhen Tang

Ning Wang

Shusen Bao

Zhigang Wu

Lei Shi

Xiongfei Zheng

Xiaokang Li

Zheng Guo

Affiliations

Soon will be listed here.

Abstract

Biodegradable polycaprolactone/β-tricalcium phosphate (PT) composites are desirable candidates for bone tissue engineering applications. A higher β-tricalcium phosphate (TCP) ceramic content improves the mechanical, hydrophilic and osteogenic properties of PT scaffolds . Using a dynamic degradation reactor, we established a steady degradation model to investigate the changes in the physio-chemical and biological properties of PT scaffolds during degradation.PT46 and PT37 scaffolds underwent degradation more rapidly than PT scaffolds with lower TCP contents. studies revealed the rapid degradation of PT (PT46 and PT37) scaffolds disturbed macrophage responses and lead to bone healing failure. Macrophage co-culture assays and a subcutaneous implantation model indicated that the scaffold degradation process dynamically affected macrophage responses, especially polarization. RNA-Seq analysis indicated phagocytosis of the degradation products of PT37 scaffolds induces oxidative stress and inflammatory M1 polarization in macrophages. Overall, this study reveals that the dynamic patterns of biodegradation of degradable bone scaffolds highly orchestrate immune responses and thus determine the success of bone regeneration. Therefore, through evaluation of the biological effects of biomaterials during the entire process of degradation on immune responses and bone regeneration are necessary in order to develop more promising biomaterials for bone regeneration.

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