A Clinical Trial About Effects of Prebiotic and Probiotic Supplementation on Weight Loss, Psychological Profile and Metabolic Parameters in Obese Subjects

Overview

Journal Endocrinol Diabetes Metab

Specialty Endocrinology

Date 2023 Jan 6

PMID 36606510

Authors

Rym Ben Othman

Nadia Ben Amor

Faten Mahjoub

Olfa Berriche

Chaima El Ghali

Amel Gamoudi

Henda Jamoussi

Affiliations

Soon will be listed here.

Abstract

Introduction: The management of obesity is difficult with many failures of lifestyle measures, hence the need to broaden the range of treatments prescribed. The aim of our work was to study the influence of pre and probiotics on weight loss psychological profile and metabolic parameters in obese patients.

Methods: It is a clinical trial involving 45 obese patients, recruited from the Obesity Unit of the National Institute of Nutrition between March and August 2022 divided into three groups: diet only (low-carbohydrate and reduced energy diet), prebiotics (30 g of carob/day) and probiotics (one tablet containing Bifidobacterium longum, Lactobacillus helveticus, Lactococcus lactis, Streptococcus thermophilus/day). The three groups were matched for age, sex and BMI. Patients were seen after 1 month from the intervention. Anthropometric measures, biological parameters, dietary survey and psychological scores were performed.

Results: The average age of our population was 48.73 ± 7.7 years, with a female predominance. All three groups showed a significant decrease in weight, BMI and waist circumference with p < .05. Only the prebiotic and probiotic group showed a significant decrease in fat mass (p = .001) and a significant increase in muscle strength with p = .008 and .004, but the differences were not significant between the three groups. Our results showed also a significant decrease in insulinemia and HOMA-IR in the prebiotic group compared to the diet-alone group (p = .03; p = .012) and the probiotic group showed a significant decrease in fasting blood glucose compared to the diet alone group (p = .02). A significant improvement in sleep quality was noted in the prebiotic group (p = .02), with a significant decrease in depression, anxiety and stress in all three groups.

Conclusions: The prescription of prebiotics and probiotics with the lifestyle measures seems interesting for the management of obesity especially if it is sarcopenic, in addition to the improvement of metabolic parameters and obesity-related psychiatric disorders.

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References

Kazemi A, Noorbala A, Azam K, Eskandari M, Djafarian K . Effect of probiotic and prebiotic vs placebo on psychological outcomes in patients with major depressive disorder: A randomized clinical trial. Clin Nutr. 2018; 38(2):522-528. DOI: 10.1016/j.clnu.2018.04.010. View

Hiel S, Gianfrancesco M, Rodriguez J, Portheault D, Leyrolle Q, Bindels L . Link between gut microbiota and health outcomes in inulin -treated obese patients: Lessons from the Food4Gut multicenter randomized placebo-controlled trial. Clin Nutr. 2020; 39(12):3618-3628. DOI: 10.1016/j.clnu.2020.04.005. View

Wu Y, Ye Z, Feng P, Li R, Chen X, Tian X . JL-3 isolated from "Jiangshui" ameliorates hyperuricemia by degrading uric acid. Gut Microbes. 2021; 13(1):1-18. PMC: 8007157. DOI: 10.1080/19490976.2021.1897211. View

Esteve E, Ricart W, Fernandez-Real J . Gut microbiota interactions with obesity, insulin resistance and type 2 diabetes: did gut microbiote co-evolve with insulin resistance?. Curr Opin Clin Nutr Metab Care. 2011; 14(5):483-90. DOI: 10.1097/MCO.0b013e328348c06d. View

Neyrinck A, Rodriguez J, Zhang Z, Seethaler B, Sanchez C, Roumain M . Prebiotic dietary fibre intervention improves fecal markers related to inflammation in obese patients: results from the Food4Gut randomized placebo-controlled trial. Eur J Nutr. 2021; 60(6):3159-3170. PMC: 8354918. DOI: 10.1007/s00394-021-02484-5. View

Parnell J, Raman M, Rioux K, Reimer R . The potential role of prebiotic fibre for treatment and management of non-alcoholic fatty liver disease and associated obesity and insulin resistance. Liver Int. 2012; 32(5):701-11. DOI: 10.1111/j.1478-3231.2011.02730.x. View

Riachy M, Juvelikian G, Sleilaty G, Bazarbachi T, Khayat G, Mouradides C . [Validation of the Arabic Version of the Epworth Sleepiness Scale: Multicentre study]. Rev Mal Respir. 2012; 29(5):697-704. DOI: 10.1016/j.rmr.2011.12.017. View

FRIEDEWALD W, Levy R, Fredrickson D . Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972; 18(6):499-502. View

Suzumura E, Bersch-Ferreira A, Torreglosa C, da Silva J, Coqueiro A, Kuntz M . Effects of oral supplementation with probiotics or synbiotics in overweight and obese adults: a systematic review and meta-analyses of randomized trials. Nutr Rev. 2019; 77(6):430-450. DOI: 10.1093/nutrit/nuz001. View

10.

Fassatoui M, Lopez-Siles M, Diaz-Rizzolo D, Jmel H, Naouali C, Abdessalem G . Gut microbiota imbalances in Tunisian participants with type 1 and type 2 diabetes mellitus. Biosci Rep. 2019; 39(6). PMC: 6579978. DOI: 10.1042/BSR20182348. View

11.

Saleh-Ghadimi S, Dehghan P, Sarmadi B, Maleki P . Improvement of sleep by resistant dextrin prebiotic in type 2 diabetic women coincides with attenuation of metabolic endotoxemia: involvement of gut-brain axis. J Sci Food Agric. 2022; 102(12):5229-5237. DOI: 10.1002/jsfa.11876. View

12.

Cerdo T, Garcia-Santos J, Bermudez M, Campoy C . The Role of Probiotics and Prebiotics in the Prevention and Treatment of Obesity. Nutrients. 2019; 11(3). PMC: 6470608. DOI: 10.3390/nu11030635. View

13.

Ben Othman R, Ben Amor N, Mahjoub F, Berriche O, El Ghali C, Gamoudi A . A clinical trial about effects of prebiotic and probiotic supplementation on weight loss, psychological profile and metabolic parameters in obese subjects. Endocrinol Diabetes Metab. 2023; 6(2):e402. PMC: 10000630. DOI: 10.1002/edm2.402. View

14.

Sergeev I, Aljutaily T, Walton G, Huarte E . Effects of Synbiotic Supplement on Human Gut Microbiota, Body Composition and Weight Loss in Obesity. Nutrients. 2020; 12(1). PMC: 7019807. DOI: 10.3390/nu12010222. View

15.

Terkawi A, Tsang S, AlKahtani G, Al-Mousa S, Al Musaed S, AlZoraigi U . Development and validation of Arabic version of the Hospital Anxiety and Depression Scale. Saudi J Anaesth. 2017; 11(Suppl 1):S11-S18. PMC: 5463562. DOI: 10.4103/sja.SJA_43_17. View

16.

K Stenman L, Lehtinen M, Meland N, Christensen J, Yeung N, Saarinen M . Probiotic With or Without Fiber Controls Body Fat Mass, Associated With Serum Zonulin, in Overweight and Obese Adults-Randomized Controlled Trial. EBioMedicine. 2016; 13:190-200. PMC: 5264483. DOI: 10.1016/j.ebiom.2016.10.036. View

17.

Bakker G, Meijnikman A, Scheithauer T, Davids M, Aydin O, Boerlage T . Fecal microbiota transplantation does not alter bacterial translocation and visceral adipose tissue inflammation in individuals with obesity. Obes Sci Pract. 2022; 8(1):56-65. PMC: 8804924. DOI: 10.1002/osp4.545. View

18.

Quero C, Manonelles P, Fernandez M, Abellan-Aynes O, Lopez-Plaza D, Andreu-Caravaca L . Differential Health Effects on Inflammatory, Immunological and Stress Parameters in Professional Soccer Players and Sedentary Individuals after Consuming a Synbiotic. A Triple-Blinded, Randomized, Placebo-Controlled Pilot Study. Nutrients. 2021; 13(4). PMC: 8073688. DOI: 10.3390/nu13041321. View

19.

Kanazawa A, Aida M, Yoshida Y, Kaga H, Katahira T, Suzuki L . Effects of Synbiotic Supplementation on Chronic Inflammation and the Gut Microbiota in Obese Patients with Type 2 Diabetes Mellitus: A Randomized Controlled Study. Nutrients. 2021; 13(2). PMC: 7914668. DOI: 10.3390/nu13020558. View

20.

Zhao J, Huang Y, Yu X . A Narrative Review of Gut-Muscle Axis and Sarcopenia: The Potential Role of Gut Microbiota. Int J Gen Med. 2021; 14:1263-1273. PMC: 8053521. DOI: 10.2147/IJGM.S301141. View