Gut Colonisation with Multidrug-resistant Klebsiella Pneumoniae Worsens Pseudomonas Aeruginosa Lung Infection

Overview

Journal Nat Commun

Specialty Biology

Date 2023 Jan 5

PMID 36604442

Authors

Remi Le Guern

Teddy Grandjean

Sarah Stabler

Marvin Bauduin

Philippe Gosset

Eric Kipnis

Rodrigue Dessein

Affiliations

Soon will be listed here.

Abstract

Carbapenemase-producing Enterobacterales (CPE) are spreading rapidly in hospital settings. Asymptomatic CPE gut colonisation may be associated with dysbiosis and gut-lung axis alterations, which could impact lung infection outcomes. In this study, in male C57BL/6JRj mice colonised by CPE, we characterise the resulting gut dysbiosis, and analyse the lung immune responses and outcomes of subsequent Pseudomonas aeruginosa lung infection. Asymptomatic gut colonisation by CPE leads to a specific gut dysbiosis and increases the severity of P. aeruginosa lung infection through lower numbers of alveolar macrophages and conventional dendritic cells. CPE-associated dysbiosis is characterised by a near disappearance of the Muribaculaceae family and lower levels of short-chain fatty acids. Faecal microbiota transplantation restores immune responses and outcomes of lung infection outcomes, demonstrating the involvement of CPE colonisation-induced gut dysbiosis in altering the immune gut-lung axis, possibly mediated by microbial metabolites such as short-chain fatty acids.

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