Bovine Neutrophil β-Defensin-5 Provides Protection Against Multidrug-Resistant Via Regulating Pulmonary Inflammatory Response and Metabolic Response

(), a kind of zoonotic bacteria, is among the most common antibiotic-resistant pathogens, and it causes nosocomial infections that pose a threat to public health. In this study, the roles of synthetic bovine neutrophil β-defensin-5 (B5) in regulating inflammatory response and metabolic response against multidrug-resistant infection in a mouse model were investigated. Mice were administrated intranasally with 20 μg of B5 twice and challenged with three days after B5 pretreatment. Results showed that B5 failed to directly kill in vitro, but it provided effective protection against multidrug-resistant via decreasing the bacterial load in the lungs and spleen, and by alleviating -induced histopathological damage in the lungs. Furthermore, B5 significantly enhanced the mRNA expression of TNF-α, IL-1β, Cxcl1, Cxcl5, Ccl17, and Ccl22 and obviously enhanced the rapid recruitment of macrophages and dendritic cells in the lungs in the early infection phase, but significantly down-regulated the levels of TNF-α, IL-1β, and IL-17 in the lungs in the later infection phase. Moreover, RNA-seq results showed that infection activated signaling pathways related to natural killer cell-mediated cytotoxicity, IL-17 signaling pathway, inflammatory response, apoptosis, and necroptosis in the lungs, while B5 inhibited these signaling pathways. Additionally, challenge led to the suppression of glycerophospholipid metabolism, the phosphotransferase system, the activation of microbial metabolism in diverse environments, and metabolic pathways in the lungs. However, B5 significantly reversed these metabolic responses. Collectively, B5 can effectively regulate the inflammatory response caused by and offer protection against . B5 may be applied as an adjuvant to the existing antimicrobial therapy to control multidrug-resistant infection. Our study highlights the potential of B5 in enhancing pulmonary bacterial clearance and alleviating -caused inflammatory damage.