Occurrence of Campylobacter Spp. and Phenotypic Antimicrobial Resistance Profiles of Campylobacter Jejuni in Slaughtered Broiler Chickens in North-Western Romania

Overview

Journal Antibiotics (Basel)

Specialty Pharmacology

Date 2022 Dec 23

PMID 36551369

Authors

Sebastian Alexandru Popa

Adriana Morar

Alexandra Ban-Cucerzan

Emil Tirziu

Viorel Herman

Khalid Ibrahim Sallam

Doru Morar

Ulas Acaroz

Mirela Imre

Tijana Florea

Hamid Mukhtar

Kalman Imre

Affiliations

Soon will be listed here.

Abstract

Campylobacteriosis is recognized as one of the most common food-borne zoonoses, with worldwide distribution, having undercooked poultry meat and other cross-contaminated foodstuffs as the main sources of human infections. The current study aimed to provide data on the occurrence of the thermophilic spp. in seven broiler chicken flocks, from three north-western Transylvanian counties of Romania, as well as to determine the antimicrobial resistance profile of the isolated strains. A total of 324 fresh cecal samples were collected during the slaughtering process, and screened for the presence of spp., using routine microbiological and molecular diagnostic tools. Overall, 85.2% (276/324; 95% CI 80.9-88.6) of the tested samples expressed positive results for spp., with dominant occurrence of towards (63.4% vs. 36.6%). From the six tested antimicrobials, the 101 isolated strains were resistant against ciprofloxacin (79.2%), nalidixic acid (78.2%), tetracycline (49.5%), and streptomycin (7.9%), but total susceptibility was noticed against erythromycin and gentamicin. Seven (6.9%) isolates exhibited multidrug resistance. The study results emphasize the role of broiler chicken as reservoir of infections for humans, as well as strengthen the necessity of the prudent using of antimicrobials in the poultry industry.

Citing Articles

A Decade of Antimicrobial Resistance in Human and Animal spp. Isolates.

Barata R, Saavedra M, Almeida G Antibiotics (Basel). 2024; 13(9).

PMID: 39335077 PMC: 11429304. DOI: 10.3390/antibiotics13090904.

Molecular Detection of Virulence-Associated Markers in and Isolates From Water, Cattle, and Chicken Faecal Samples From Kajiado County, Kenya.

Wanja D, Mbindyo C, Mbuthia P, Bebora L, Aboge G Biomed Res Int. 2024; 2024:4631351.

PMID: 39166218 PMC: 11335418. DOI: 10.1155/2024/4631351.

First study in the frequency of isolation and phenotypic antimicrobial resistance profiles of pig and cattle origin Campylobacter strains in Romania.

Popa S, Morar A, Ban-Cucerzan A, Tirziu E, Herman V, Imre M Vet Res Commun. 2024; 48(4):2621-2627.

PMID: 38556573 DOI: 10.1007/s11259-024-10360-w.

Detection of Viable but Non-Culturable (VBNC)- in the Environment of Broiler Farms: Innovative Insights Delivered by Propidium Monoazide (PMA)-v-qPCR Analysis.

Reichelt B, Szott V, Stingl K, Roesler U, Friese A Microorganisms. 2023; 11(10).

PMID: 37894150 PMC: 10609165. DOI: 10.3390/microorganisms11102492.

The Silent Threat: Antimicrobial-Resistant Pathogens in Food-Producing Animals and Their Impact on Public Health.

Almansour A, Alhadlaq M, Alzahrani K, Mukhtar L, AlHarbi A, Alajel S Microorganisms. 2023; 11(9).

PMID: 37763971 PMC: 10537193. DOI: 10.3390/microorganisms11092127.

References

Natsos G, Mouttotou N, Magiorkinis E, Ioannidis A, Rodi-Burriel A, Chatzipanagiotou S . Prevalence of and Risk Factors for spp. Colonization of Broiler Chicken Flocks in Greece. Foodborne Pathog Dis. 2020; 17(11):679-686. DOI: 10.1089/fpd.2020.2795. View

Kalupahana R, Mughini-Gras L, Kottawatta S, Somarathne S, Gamage C, Wagenaar J . Weather correlates of Campylobacter prevalence in broilers at slaughter under tropical conditions in Sri Lanka. Epidemiol Infect. 2018; 146(8):972-979. PMC: 9184945. DOI: 10.1017/S0950268818000894. View

Giombelli A, Gloria M . Prevalence of Salmonella and Campylobacter on broiler chickens from farm to slaughter and efficiency of methods to remove visible fecal contamination. J Food Prot. 2014; 77(11):1851-9. DOI: 10.4315/0362-028X.JFP-14-200. View

Sillero M, Almirall J . Campylobacter jejuni and hemolytic-uremic syndrome. Nephron. 1999; 82(4):363-4. DOI: 10.1159/000045457. View

Gharbi M, Bejaoui A, Ben Hamda C, Jouini A, Ghedira K, Zrelli C . Prevalence and Antibiotic Resistance Patterns of spp. Isolated from Broiler Chickens in the North of Tunisia. Biomed Res Int. 2019; 2018:7943786. PMC: 6323509. DOI: 10.1155/2018/7943786. View

Dan S, Tabaran A, Mihaiu L, Mihaiu M . Antibiotic susceptibility and prevalence of foodborne pathogens in poultry meat in Romania. J Infect Dev Ctries. 2015; 9(1):35-41. DOI: 10.3855/jidc.4958. View

. The European Union One Health 2019 Zoonoses Report. EFSA J. 2021; 19(2):e06406. PMC: 7913300. DOI: 10.2903/j.efsa.2021.6406. View

Agunos A, Waddell L, Leger D, Taboada E . A systematic review characterizing on-farm sources of Campylobacter spp. for broiler chickens. PLoS One. 2014; 9(8):e104905. PMC: 4149356. DOI: 10.1371/journal.pone.0104905. View

Butzler J . Campylobacter, from obscurity to celebrity. Clin Microbiol Infect. 2004; 10(10):868-76. DOI: 10.1111/j.1469-0691.2004.00983.x. View

10.

Sallam K, Abd-Elghany S, Imre K, Morar A, Herman V, Hussein M . Ensuring safety and improving keeping quality of meatballs by addition of sesame oil and sesamol as natural antimicrobial and antioxidant agents. Food Microbiol. 2021; 99:103834. DOI: 10.1016/j.fm.2021.103834. View

11.

Greige S, Rivoal K, Osman M, El Safadi D, Dabboussi F, El Hage R . Prevalence and genetic diversity of Campylobacter spp. in the production chain of broiler chickens in Lebanon and its association with the intestinal protozoan Blastocystis sp. Poult Sci. 2019; 98(11):5883-5891. DOI: 10.3382/ps/pez286. View

12.

Vinueza-Burgos C, Wautier M, Martiny D, Cisneros M, Van Damme I, De Zutter L . Prevalence, antimicrobial resistance and genetic diversity of Campylobacter coli and Campylobacter jejuni in Ecuadorian broilers at slaughter age. Poult Sci. 2017; 96(7):2366-2374. PMC: 5850218. DOI: 10.3382/ps/pew487. View

13.

Allen V, Bull S, Corry J, Domingue G, Jorgensen F, Frost J . Campylobacter spp. contamination of chicken carcasses during processing in relation to flock colonisation. Int J Food Microbiol. 2006; 113(1):54-61. DOI: 10.1016/j.ijfoodmicro.2006.07.011. View

14.

Iannetti S, Calistri P, Serafino G, Marotta F, Alessiani A, Antoci S . Campylobacter jejuni and Campylobacter coli: prevalence, contamination levels, genetic diversity and antibiotic resistance in Italy. Vet Ital. 2020; 56(1). DOI: 10.12834/VetIt.1819.9596. View

15.

Silva J, Leite D, Fernandes M, Mena C, Gibbs P, Teixeira P . Campylobacter spp. as a Foodborne Pathogen: A Review. Front Microbiol. 2011; 2:200. PMC: 3180643. DOI: 10.3389/fmicb.2011.00200. View

16.

Allos B . Campylobacter jejuni Infections: update on emerging issues and trends. Clin Infect Dis. 2001; 32(8):1201-6. DOI: 10.1086/319760. View

17.

. The European Union Summary Report on Antimicrobial Resistance in zoonotic and indicator bacteria from humans, animals and food in 2018/2019. EFSA J. 2021; 19(4):e06490. PMC: 8040295. DOI: 10.2903/j.efsa.2021.6490. View

18.

Fraqueza M, Martins A, Borges A, Fernandes M, Fernandes M, Vaz Y . Antimicrobial resistance among Campylobacter spp. strains isolated from different poultry production systems at slaughterhouse level. Poult Sci. 2014; 93(6):1578-86. DOI: 10.3382/ps.2013-03729. View

19.

Nylen G, Dunstan F, Palmer S, Andersson Y, Bager F, Cowden J . The seasonal distribution of campylobacter infection in nine European countries and New Zealand. Epidemiol Infect. 2002; 128(3):383-90. PMC: 2869833. DOI: 10.1017/s0950268802006830. View

20.

Imre K, Herman V, Morar A . Scientific Achievements in the Study of the Occurrence and Antimicrobial Susceptibility Profile of Major Foodborne Pathogenic Bacteria in Foods and Food Processing Environments in Romania: Review of the Last Decade. Biomed Res Int. 2020; 2020:5134764. PMC: 7333035. DOI: 10.1155/2020/5134764. View