Prevalence and Antibiotic Resistance Patterns of Spp. Isolated from Broiler Chickens in the North of Tunisia

Overview

Journal Biomed Res Int

Publisher Wiley

Specialties Biomedical Engineering
Biotechnology
General Medicine

Date 2019 Jan 24

PMID 30671471

Citations 24

Authors

Manel Gharbi

Awatef Bejaoui

Cherif Ben Hamda

Ahlem Jouini

Kais Ghedira

Chahrazed Zrelli

Safa Hamrouni

Chedia Aouadhi

Ghaith Bessoussa

Abdeljelil Ghram

Abderrazek Maaroufi

Affiliations

Soon will be listed here.

Abstract

The aim of the current study is to assess the prevalence of infection in broiler chickens, raised in intensive production conditions, and to evaluate the antimicrobial susceptibility of recovered isolates. A total of 590 cloacal swab samples were taken from 13 broiler chicken flocks in the North East of Tunisia. All samples were tested for the presence of thermophilic by culture and PCR, targeting the and genes, respectively. Susceptibility to antimicrobial drugs was tested against 8 antibiotics. Prevalence of infection, relationship with geographic origins and seasons, antimicrobial resistance rates and patterns were analyzed. Total prevalence of infection in broiler flocks was in the range of 22.4%, with a predominance of (68.9%), followed by (31.1%). Positive association was highlighted between the infection level and the season ( < 0.001), but no link was emphasized considering the geographic origin. Antimicrobial susceptibility testing revealed very high resistance rates detected against macrolide, tetracycline, quinolones, and chloramphenicol, ranging from 88.6% to 100%. Lower resistance prevalence was noticed for -lactams (47% and 61.4%) and gentamicin (12.9%). 17 R-type patterns were observed, and a common pattern was found in 30.3% of isolates. This study provides updates and novel data on the prevalence and the AMR of broiler campylobacters in Tunisia, revealing the occurrence of high resistance to several antibiotics and emphasizing the requirement of better surveillance and careful regulation of antimicrobials use.

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