Network Pharmacology Approach and Experimental Verification of Dan-Shen Decoction in the Treatment of Ischemic Heart Disease

Overview

Journal Pharm Biol

Specialties Pharmacology
Pharmacy

Date 2022 Dec 22

PMID 36546685

Authors

Difei Gong

Tianyi Yuan

Ranran Wang

Shuchan Sun

Awaguli Dawuti

Shoubao Wang

Guanhua Du

Lianhua Fang

Affiliations

Soon will be listed here.

Abstract

Context: Dan-Shen Decoction, which is composed of Danshen, Tanxiang and Sharen, has a good therapeutic effect on ischemic heart disease (IHD). However, systematic research on the exact mechanism of action of Dan-Shen Decoction is still lacking. The anti-IHD effect of Dan-Shen Decoction was examined in this study using a systematic pharmacological method.

Objective: This study validates the efficacy and explores the potential mechanisms of Dan-Shen Decoction in treating IHD by integrating network pharmacology analyses and experimental verification.

Materials And Methods: The active components, critical targets and potential mechanisms of Dan-Shen Decoction against IHD were predicted by network pharmacology and molecule docking. H9c2 cells were pretreated with various 1 µg/mL Dan-Shen Decoction for 2 h before induction with 1000 µmol/L CoCl for 24 h. The cell viability was detected by CCK8, and protein expression was detected by western blots.

Results: The network pharmacology approach successfully identified 69 active components in Dan-Shen Decoction, and 122 potential targets involved in the treatment of IHD. The experiments indicate that the anti-IHD effect of Dan-Shen Decoction may be closely associated with targets such as AKT1 and MAPK1, as well as biological processes such as cell proliferation, inflammatory response, and metabolism.

Conclusions: This study not only provides new insights into the mechanism of Dan-Shen Decoction against IHD, but also provides important information and new research ideas for the discovery of anti-IHD compounds from traditional Chinese medicine.

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